Product nameAnti-p38 antibody
See all p38 primary antibodies
DescriptionRabbit polyclonal to p38
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Sheep, Cow, Dog, Chimpanzee
Recombinant full length protein corresponding to Human p38 MAPK.
Database link: Q16539
- WB: HEK-293T, A431 and HeLa whole cell lysate. IHC: Rat brain and human lung adenocarcinoma tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 41 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionResponds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
Tissue specificityBrain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- CSAID Binding Protein 1 antibody
- CSAID binding protein antibody
- CSAID-binding protein antibody
All lanes : Anti-p38 antibody (ab227426) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Paraffin-embedded rat brain tissue stained for p38 using ab227426 at 1/500 in immunohistochemical analysis.
Paraffin-embedded human lung adenocarcinoma tissue stained for p38 using ab227426 at 1/250 in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for p38 (green) using ab227426 at 1/100 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde for 10 minutes. Blue: Hoechst 33342 staining.
ab227426 has not yet been referenced specifically in any publications.