Overview

  • Product name

    Anti-p38 antibody [Y122] - BSA and Azide free
    See all p38 primary antibodies
  • Description

    Rabbit monoclonal [Y122] to p38 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of human p38.

  • Positive control

    • WB: Hela, Jurkat, K562 and MCF7 cell lyastes. IHC-P: Human skin carcinoma. ICC/IF: MCF7 cells.
  • General notes

    Ab227995 is the carrier-free version of ab32142. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab227995 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab227995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
    • Tissue specificity

      Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • Domain

      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      modifications

      Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • CSAID Binding Protein 1 antibody
      • CSAID binding protein antibody
      • CSAID-binding protein antibody
      • Csaids binding protein antibody
      • CSBP 1 antibody
      • CSBP 2 antibody
      • CSBP antibody
      • CSBP1 antibody
      • CSBP2 antibody
      • CSPB1 antibody
      • Cytokine suppressive anti-inflammatory drug-binding protein antibody
      • EXIP antibody
      • MAP kinase 14 antibody
      • MAP kinase MXI2 antibody
      • MAP kinase p38 alpha antibody
      • MAPK 14 antibody
      • MAPK14 antibody
      • MAX interacting protein 2 antibody
      • MAX-interacting protein 2 antibody
      • Mitogen Activated Protein Kinase 14 antibody
      • Mitogen activated protein kinase p38 alpha antibody
      • Mitogen-activated protein kinase 14 antibody
      • Mitogen-activated protein kinase p38 alpha antibody
      • MK14_HUMAN antibody
      • Mxi 2 antibody
      • MXI2 antibody
      • p38 ALPHA antibody
      • p38 antibody
      • p38 MAP kinase antibody
      • p38 MAPK antibody
      • p38 mitogen activated protein kinase antibody
      • p38ALPHA antibody
      • p38alpha Exip antibody
      • PRKM14 antibody
      • PRKM15 antibody
      • RK antibody
      • SAPK2A antibody
      • Stress-activated protein kinase 2a antibody
      see all

    Images

    • This WB data was generated using the same anti-p38 antibody clone, Y122, in a different buffer formulation (cat# ab32142).

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: p38 knockout HAP1 cell lysate (20 µg) 
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg) 
      Lanes 1 - 4: Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32142 was shown to specifically react with p38 when p38 knockout samples were used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab32142 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)  secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling p38 (green) with purified ab32142 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32142).

    • This IHC data was generated using the same anti-p38 antibody clone, Y122, in a different buffer formulation (cat# ab32142).

      Ab32142, at a 1/100 dilution, staining p38 in paraffin embedded human skin carcinoma tissue by immunohistochemistry.

    References

    This product has been referenced in:

    • Fang Y  et al. Inactivation of p38 MAPK contributes to stem cell-like properties of non-small cell lung cancer. Oncotarget 8:26702-26717 (2017). WB . Read more (PubMed: 28460458) »
    • Zhang J  et al. Comparisons of ethanol extracts of chinese propolis (poplar type) and poplar gums based on the antioxidant activities and molecular mechanism. Evid Based Complement Alternat Med 2015:307594 (2015). Mouse . Read more (PubMed: 25802536) »
    See all 8 Publications for this product

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