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    p38-deltamapk13--p38-alphamapk14-antibody-m138-ab31828.pdf

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Validated using a knockout cell line

Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)

  • Datasheet
  • SDS
Reviews (15)Q&A (2)References (109)

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Western blot - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
  • Western blot - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
  • Western blot - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
  • Western blot - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
  • Immunocytochemistry/ Immunofluorescence - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
  • Flow Cytometry - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
  • Immunocytochemistry/ Immunofluorescence - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)

Key features and details

  • Mouse monoclonal [M138] to p38 delta/MAPK13 + p38 alpha/MAPK14
  • Suitable for: IHC-P, Flow Cyt, WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138]
  • Description

    Mouse monoclonal [M138] to p38 delta/MAPK13 + p38 alpha/MAPK14
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Rat p38 alpha/MAPK14 (C terminal). Identical to human and mouse.

  • Epitope

    ab31828 recognises an epitope located in the C terminal region of p38.
  • Positive control

    • A431 cells, Jurkat cells, HeLa cells. IHC: Human esophagus (FFPE)
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified with protein A affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    M138
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • MAPK pathway
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Kits/ Lysates/ Other
    • Kits
    • Cell Signaling Kits
    • Other Cell Signaling Kits

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant human p38 alpha/MAPK14 protein (ab82188)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab31828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/20 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB (13)
1/1000. Predicted molecular weight: 41 kDa.
ICC/IF (2)
1/200.
Notes
IHC-P
1/20 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
1/1000. Predicted molecular weight: 41 kDa.
ICC/IF
1/200.

Target

  • Cellular localization

    p38 alpha/MAPK14: Cytoplasm. Nucleus.
  • Database links

    • Entrez Gene: 1432 Human
    • Entrez Gene: 5603 Human
    • Entrez Gene: 26415 Mouse
    • Entrez Gene: 26416 Mouse
    • Omim: 600289 Human
    • Omim: 602899 Human
    • SwissProt: O15264 Human
    • SwissProt: Q16539 Human
    • SwissProt: P47811 Mouse
    • SwissProt: Q9Z1B7 Mouse
    • Unigene: 178695 Human
    • Unigene: 485233 Human
    • Unigene: 27970 Mouse
    • Unigene: 311337 Mouse
    see all

Images

  • Western blot - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
    Western blot - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
    All lanes : Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828) at 1/1000 dilution

    Lane 1 : Recombinant Human p38 beta/MAPK11 protein (ab117219)
    Lane 2 : Recombinant Human p38 gamma/MAPK12 protein (ab117221)
    Lane 3 : Recombinant Human p38 delta/MAPK13 protein (ab113869)
    Lane 4 : Recombinant human p38 alpha/MAPK14 protein (ab82188)

    Predicted band size: 41 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)

    IHC image of ab31828 staining p38 in normal human esophagus formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31828, 1/50 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
    Western blot - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: p38 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab31828 observed at 40 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab31828 was shown to specifically react with p38 when p38 knockout samples were used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab31828 and ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
    Western blot - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
    Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38alpha (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phoshpo-p38 (T180/Y182) peptide (lane 7).
  • Western blot - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
    Western blot - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: p38 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab31828 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This western blot image is a comparison between ab31828 and a competitor's top cited rabbit polyclonal antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
    Immunocytochemistry/ Immunofluorescence - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)

    Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse with ab31828. The cells were labeled with mouse monoclonal p38α MAPK and p38 MAPK antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

  • Flow Cytometry - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
    Flow Cytometry - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)

    Overlay histogram showing A431 cells stained with ab31828 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab21828, 1:100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)
    Immunocytochemistry/ Immunofluorescence - Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828)This image is courtesy of an anonymous Abreview.

    Immunocytochemical analysis of mouse embryo fibroblast cells (NIH-3T3), labelling p38 with ab31828. Sample fixed in paraformaldehyde and blocked with 1% Donkey Serum + 1% BSA + 0.1% Triton X-100, in PBS for 30 minutes at 22°C. Incubated with ab31828 diluted 1/500 in 1% Donkey Serum + 1% BSA + 0.1% Triton X for 1 hour at 22°C. Secondary antibody was a Donkey anti-Mouse polyclonal conjugated to Alexa Fluor® 488, diluted 1/500. 

    See Abreview

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (109)

Publishing research using ab31828? Please let us know so that we can cite the reference in this datasheet.

ab31828 has been referenced in 109 publications.

  • Li Y  et al. LncRNA-LET relieves hypoxia-induced injury in H9c2 cells through regulation of miR-138. J Cell Biochem 121:259-268 (2020). PubMed: 31222827
  • Qi S  et al. P38 MAPK signaling pathway mediates COM crystal-induced crystal adhesion change in rat renal tubular epithelial cells. Urolithiasis 48:9-18 (2020). PubMed: 31183507
  • Liu D  et al. microRNA-155 Modulates Hepatic Stellate Cell Proliferation, Apoptosis, and Cell Cycle Progression in Rats With Alcoholic Hepatitis via the MAPK Signaling Pathway Through Targeting SOCS1. Front Pharmacol 11:270 (2020). PubMed: 32317960
  • Deng QF  et al. Cyclooxygenase-2 mediates gefitinib resistance in non-small cell lung cancer through the EGFR/PI3K/AKT axis. J Cancer 11:3667-3674 (2020). PubMed: 32284763
  • Xi J  et al. miR-155 inhibition represents a potential valuable regulator in mitigating myocardial hypoxia/reoxygenation injury through targeting BAG5 and MAPK/JNK signaling. Mol Med Rep 21:1011-1020 (2020). PubMed: 31922242
View all Publications for this product

Customer reviews and Q&As

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1-10 of 17 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-p38 antibody [M138]

Poor
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Equus Cell (Tenocytes)
Permeabilization
Yes - 0.1% Tritin-X in PBS
Specification
Tenocytes
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Ross Beaumont

Verified customer

Submitted Mar 18 2020

Western blot abreview for Anti-p38 antibody [M138]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human tissue)
Gel Running Conditions
Reduced Denaturing (10% Acrylamide gel)
Loading amount
20 µg
Treatment
UV B 100mJ/cm2
Specification
Human tissue
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Mr. Jeong-Yong Park

Verified customer

Submitted Apr 25 2019

Western blot abreview for Anti-p38 antibody [M138]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - other (muscle)
Gel Running Conditions
Reduced Denaturing (9)
Loading amount
40 µg
Specification
muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Nov 01 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-p38 antibody [M138]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH-3T3 cells)
Permeabilization
No
Specification
NIH-3T3 cells
Blocking step
1% Donkey Serum + 1% BSA + 0.1% Triton X-100, in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 02 2015

Western blot abreview for Anti-p38 antibody [M138]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - other (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Aug 25 2014

Western blot abreview for Anti-p38 antibody [M138]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Mouse Tissue lysate - whole (Mouse whole embryo (E10.5) extract)
Specification
Mouse whole embryo (E10.5) extract
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Aug 25 2014

Western blot abreview for Anti-p38 [M138] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Syrian Hamster Cell lysate - whole cell (BHK cells)
Loading amount
100 µg
Specification
BHK cells
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted May 14 2013

Western blot abreview for Anti-p38 [M138] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (COS cells)
Loading amount
100 µg
Specification
COS cells
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted May 10 2013

Western blot abreview for Anti-p38 [M138] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
100 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted May 07 2013

Western blot abreview for Anti-p38 [M138] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Cow Cell lysate - whole cell (MDBK cells)
Loading amount
100 µg
Specification
MDBK cells
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Apr 10 2013

1-10 of 17 Abreviews or Q&A

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