Recombinant Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)
![Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)](https://www.abcam.com/ps/products/205/ab205926/Images/ab205926-412591-anti-mapk-12-antibody-epr6528n-western-blot-human-knockout-lysate.jpg "Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6528(N)] to p38 gamma/MAPK12
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-p38 gamma/MAPK12 antibody [EPR6528(N)]
See all p38 gamma/MAPK12 primary antibodies -
Description
Rabbit monoclonal [EPR6528(N)] to p38 gamma/MAPK12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human skeletal muscle lysate; HEK-293T, HeLa, K562, C6 and PC-12 whole cell lysates; Rat heart, spleen and muscle lysates. ICC/IF: A673 and K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6528(N) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
|
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| ICC/IF |
1/500.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
|
ICC/IF
1/500. |
Target
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Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. -
Tissue specificity
Highly expressed in skeletal muscle and heart. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. -
Cellular localization
Cytoplasm. Mitochondrion. Mitochondrial when associated with SH3BP5. - Information by UniProt
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Database links
- Entrez Gene: 6300 Human
- Entrez Gene: 60352 Rat
- Omim: 602399 Human
- SwissProt: P53778 Human
- SwissProt: Q63538 Rat
- Unigene: 432642 Human
- Unigene: 162968 Rat
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Alternative names
- ERK 6 antibody
- ERK-6 antibody
- ERK6 antibody
see all
Images
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Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)All lanes : Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : MAPK12 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Human skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDaLanes 1-3: Merged signal (red and green). Green - ab205926 observed at 42 kDa. Red - loading control ab8245 observed at 36 kDa.
ab205926 Anti-MAPK 12 antibody [EPR6528(N)] was shown to specifically react with MAPK 12 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266280 (knockout cell lysate ab258041) was used. Wild-type and MAPK 12 knockout samples were subjected to SDS-PAGE. ab205926 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)](https://www.abcam.com/ps/products/205/ab205926/Images/ab205926-250160-anti-mapk-12-antibody-epr6528n-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A673 (Human muscle Ewing's Sarcoma cell line) cells labeling MAPK 12 with ab205926 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on A673 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab205926 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
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![Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)](https://www.abcam.com/ps/products/205/ab205926/Images/ab205926-276557-anti-mapk-12-antibody-epr6528n-western-blot.jpg)
Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)Lane 1: Wild type HAP1 whole cell lysate (0 µg)
Lane 2: empty knockout HAP1 whole cell lysate (20 µg)
Lane 3: MAPK 12 whole cell lysate (0 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab205926 observed at 45 kDa. Red - loading control, ab18058, observed at 124 kDa. -
![Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)](https://www.abcam.com/ps/products/205/ab205926/Images/ab205926-250170-anti-mapk-12-antibody-epr6528n-western-blot.jpg)
Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)All lanes : Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926) at 1/10000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human fetal skin lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)](https://www.abcam.com/ps/products/205/ab205926/Images/ab205926-250163-anti-mapk-12-antibody-epr6528n-western-blot.jpg)
Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)All lanes : Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)](https://www.abcam.com/ps/products/205/ab205926/Images/ab205926-250161-anti-mapk-12-antibody-epr6528n-western-blot.jpg)
Western blot - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)All lanes : Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926) at 1/1000 dilution
Lane 1 : Rat heart lysate
Lane 2 : Rat spleen lysate
Lane 3 : C6 (Rat glial tumor cells) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 5 : Rat muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 and 2: 3 minutes; Lane 3 and 4: 1 minute; Lane 5: 30 seconds
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![Immunocytochemistry/ Immunofluorescence - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)](https://www.abcam.com/ps/products/205/ab205926/Images/ab205926-250159-anti-mapk-12-antibody-epr6528n-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling MAPK 12 with ab205926 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on K562 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab205926 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
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![Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)](https://www.abcam.com/ps/products/205/ab205926/Images/ab205926-9-benefits-of-recombinant-antibodies.png)
Anti-p38 gamma/MAPK12 antibody [EPR6528(N)] (ab205926)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (3)
ab205926 has been referenced in 3 publications.
- Zhang XH et al. Targeting the non-ATP-binding pocket of the MAP kinase p38? mediates a novel mechanism of cytotoxicity in cutaneous T-cell lymphoma (CTCL). FEBS Lett 595:2570-2592 (2021). PubMed: 34455585
- Chen Q et al. YY1 targets tubulin polymerisation-promoting protein to inhibit migration, invasion and angiogenesis in pancreatic cancer via p38/MAPK and PI3K/AKT pathways. Br J Cancer 121:912-921 (2019). PubMed: 31631174
- Zhang XH et al. Multi-Kinase Inhibitor with Anti-p38? Activity in Cutaneous T-Cell Lymphoma. J Invest Dermatol N/A:N/A (2018). PubMed: 29758280
