Anti-p38 (phospho T180 + Y182) antibody (ab38238)
- Datasheet
- Specific References (9)
- Protocols
Overview
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Product nameAnti-p38 (phospho T180 + Y182) antibody
See all p38 primary antibodies -
DescriptionRabbit polyclonal to p38 (phospho T180 + Y182)
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human p38, phosphorylated at T180 and phosphorylated at Y182.
(Peptide available as ab58397.)
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Positive control
- This antibody gave a positive signal in HeLa Whole Cell Lysate and Irradiated HeLa Whole Cell Lysate (data not shown)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab38238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
Target
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FunctionResponds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
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Tissue specificityBrain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
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Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
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Post-translational
modificationsDually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 1432 Human
- Entrez Gene: 26416 Mouse
- Entrez Gene: 81649 Rat
- Omim: 600289 Human
- SwissProt: Q16539 Human
- SwissProt: P47811 Mouse
- SwissProt: P70618 Rat
- Unigene: 485233 Human
see all -
Alternative names
- CSAID Binding Protein 1 antibody
- CSAID binding protein antibody
- CSAID-binding protein antibody
see all
Images
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Western blot - Anti-p38 (phospho T180 + Y182) antibody (ab38238) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180 + Y182) antibody (ab38238)IHC image of p38 (phospho T180 + Y182) staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38238, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry/ Immunofluorescence - Anti-p38 (phospho T180 + Y182) antibody (ab38238)ICC/IF image of ab38238 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38238, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, and HepG2 cells at 5µg/ml.
Protocols
References
This product has been referenced in:
- Martinez-Useros J et al. The potential predictive value of DEK expression for neoadjuvant chemoradiotherapy response in locally advanced rectal cancer. BMC Cancer 18:144 (2018). IHC-P ; Human . Read more (PubMed: 29409457) »
- Ernst AK et al. Knockdown of L1CAM significantly reduces metastasis in a xenograft model of human melanoma: L1CAM is a potential target for anti-melanoma therapy. PLoS One 13:e0192525 (2018). Read more (PubMed: 29432466) »
Customer reviews and Q&As
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