Anti-p38IP antibody (ab122517)
Key features and details
- Rabbit polyclonal to p38IP
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p38IP antibody
See all p38IP primary antibodies -
Description
Rabbit polyclonal to p38IP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human p38IP aa 50-200.
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Positive control
- IHC-P: Human testis tissue, cerebral cortex, prostate tissue, rectum tissue. ICC/IF: U-2 OS cells. WB: HEK-293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by Fgf signaling and Snail (By similarity). Required for starvation-induced ATG9A trafficking during autophagy. -
Tissue specificity
Highly expressed in testis, moderately in brain and pituitary gland. Expressed in several fetal tissues, including lung, brain, thymus and kidney. Expression is down-regulated in malignant prostate tissues. -
Sequence similarities
Belongs to the FAM48 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55578 Human
- Omim: 613417 Human
- SwissProt: Q8NEM7 Human
- Unigene: 435815 Human
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Alternative names
- AA667204 antibody
- AI450544 antibody
- bA421P11.4 antibody
see all
Images
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Paraffin embedded human testis tissue stained for p38IP using ab122517 at 1/500 dilution in immunohistochemical analysis.
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli. Recommended concentration of ab122517 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Lanes 2-3 : Anti-p38IP antibody (ab122517)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing p38IP with a C-terminal myc-DDK tag (~3.1kDa) -
Paraffin embedded human prostate tissue stained for p38IP using ab122517 at 1/500 dilution in immunohistochemical analysis.
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Paraffin embedded human cerebral cortex tissue stained for p38IP using ab122517 at 1/500 dilution in immunohistochemical analysis.
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Paraffin embedded human rectum tissue stained for p38IP using ab122517 at 1/500 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab122517 has been referenced in 1 publication.
- Zhao S et al. Genome-scale CRISPR-Cas9 screen reveals novel regulators of B7-H3 in tumor cells. J Immunother Cancer 10:N/A (2022). PubMed: 35768165