Recombinant Anti-p39 antibody [EPR5074] (ab124896)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5074] to p39
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-p39 antibody [EPR5074]
See all p39 primary antibodies -
Description
Rabbit monoclonal [EPR5074] to p39 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within p39. The exact sequence is proprietary.
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Positive control
- Human brain tissue; Human fetal brain, Mouse brain, Rat brain and SH-SY5Y lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5074 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Activator of CDK5/TPKII. -
Tissue specificity
Brain and neuron specific. -
Sequence similarities
Belongs to the cyclin-dependent kinase 5 activator family. - Information by UniProt
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Database links
- Entrez Gene: 8941 Human
- Entrez Gene: 12570 Mouse
- Entrez Gene: 501164 Rat
- Omim: 603764 Human
- SwissProt: Q13319 Human
- SwissProt: O35926 Mouse
- Unigene: 158460 Human
- Unigene: 153833 Mouse
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Alternative names
- CD5R2_HUMAN antibody
- CDK5 activator 2 antibody
- Cdk5r2 antibody
see all
Images
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ab124896, at 1/100 dilution, staining p39 in Paraffin-embedded Human Brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-p39 antibody [EPR5074] (ab124896) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 39 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab124896 has been referenced in 1 publication.
- Pérez-Morales J et al. Hyper-phosphorylation of Rb S249 together with CDK5R2/p39 overexpression are associated with impaired cell adhesion and epithelial-to-mesenchymal transition: Implications as a potential lung cancer grading and staging biomarker. PLoS One 13:e0207483 (2018). PubMed: 30452490