Overview

  • Product name

  • Description

    Rabbit polyclonal to p40
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 164-372 of Human p40, Uniprot Q7Z6M1.

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, Molt-4 and Raji cells; A549 cells; A549 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab137691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or Tris-EDTA buffer pH8.0
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Rab9 effector required for endosome to trans-Golgi network (TGN) transport.
  • Sequence similarities

    Contains 5 Kelch repeats.
  • Post-translational
    modifications

    Phosphorylated on Ser residues most probably by PIP5K3.
  • Cellular localization

    Cytoplasm. Endosome membrane. Interaction with PIP5K3 and subsequent phosphorylation recruits it to the endosomal membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 40 kDa Rab9 effector protein antibody
    • 8430412M01Rik antibody
    • 9530020d24rik antibody
    • AV073337 antibody
    • bA65N13.1 antibody
    • C87311 antibody
    • DKFZp686P1077 antibody
    • OTTHUMP00000022126 antibody
    • OTTMUSP00000012898 antibody
    • OTTMUSP00000012899 antibody
    • OTTMUSP00000041318 antibody
    • p40 antibody
    • RAB9 effector p40 antibody
    • Rab9 effector protein with Kelch motifs antibody
    • RAB9p40 antibody
    • RABEK_HUMAN antibody
    • RABEPK antibody
    • RABEPK protein antibody
    • RGD1310612 antibody
    • RP11 65N13.1 antibody
    • RP23-446N16.2 antibody
    see all

Images

  • Anti-p40 antibody (ab137691) at 1/1000 dilution + whole cell lysate prepared from HepG2 cells at 30 µg

    Predicted band size: 41 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab137691 at 1/200 dilution.
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab137691 at 1/100 dilution.

References

This product has been referenced in:

  • Zhao Y  et al. Isolation of circulating tumor cells in patients undergoing surgery for esophageal cancer and a specific confirmation method. Oncol Lett 17:3817-3825 (2019). Read more (PubMed: 30881502) »
  • Assani K  et al. Human Cystic Fibrosis Macrophages Have Defective Calcium-Dependent Protein Kinase C Activation of the NADPH Oxidase, an Effect Augmented by Burkholderia cenocepacia. J Immunol 198:1985-1994 (2017). Read more (PubMed: 28093527) »
See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab137691.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up