Anti-p40 - DeltaNp63 antibody (ab166857)
Key features and details
- Rabbit polyclonal to p40 - DeltaNp63
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p40 - DeltaNp63 antibody
See all p40 - DeltaNp63 primary antibodies -
Description
Rabbit polyclonal to p40 - DeltaNp63 -
Host species
Rabbit -
Specificity
ab166857 recognizes deltaNp63- a p63 isoform -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide corresponding to Human p40 - DeltaNp63 aa 5-17.
Sequence:ENNAQTQFSEPQY
Database link: Q9H3D4 -
Positive control
- Lung squamous cell carcinoma and Prostate tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
Purity
Proprietary Purification -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Differentiating between lung adenocarcinoma and squamous cell carcinoma is an important distinction with therapeutic implications. Although the most frequently recommended squamous marker p63 is extremely sensitive, it suffers from low specificity due to its reactivity in a substantial proportion of lung adenocarcinomas and other tumor types, particularly lymphomas. p40 is a relatively unknown marker that recognizes ?Np63-a p63 isoform suggested to be highly specific for squamous/basal cells. p40 is equivalent to p63 in sensitivity for squamous cell carcinoma, but it is markedly superior to p63 in specificity, which eliminates a potential pitfall of misinterpreting a p63-positive adenocarcinoma or unsuspected lymphoma as squamous cell carcinoma. These findings strongly support the routine use of p40 in place of p63 for the diagnosis of pulmonary squamous cell carcinoma. -
Database links
- SwissProt: Q9H3D4 Human
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Alternative names
- 40 kDa Rab9 effector protein antibody
- 8430412M01Rik antibody
- 9530020d24rik antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody (ab166857)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Lung squamous Cell Ca, labeling p40 - DeltaNp63 using ab166857 at a 1/50 dilution.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab166857 has been referenced in 2 publications.
- Okada Y et al. Shp2-mediated MAPK pathway regulates ?Np63 in epithelium to promote corneal innervation and homeostasis. Lab Invest 100:630-642 (2020). PubMed: 31653968
- Zhao J et al. CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients. Cell Physiol Biochem 44:2138-2146 (2017). PubMed: 29241190