Overview

  • Product name
    Anti-p40 - DeltaNp63 antibody
    See all p40 - DeltaNp63 primary antibodies
  • Description
    Rabbit polyclonal to p40 - DeltaNp63
  • Host species
    Rabbit
  • Specificity
    ab167612 detects a short form of Tumor protein 63 (UniProt ID Q9H3D4) - p40 - DeltaNp63.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human p40 - DeltaNp63 aa 5-17. Epitope/Antigen: amino acids 5-17 of p40 (deltaNp63 isoforms).
    Sequence:

    ENNAQTQFSEPQY


    Database link: Q9H3D4

  • Positive control
    • IHC-P: Human lung squamous cell carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab167612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 29637001
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance
    Differentiating between lung adenocarcinoma and squamous cell carcinoma is an important distinction with therapeutic implications. Although the most frequently recommended squamous marker p63 is extremely sensitive, it suffers from low specificity due to its reactivity in a substantial proportion of lung adenocarcinomas and other tumor types, particularly lymphomas. p40 is a relatively unknown marker that recognizes ?Np63-a p63 isoform suggested to be highly specific for squamous/basal cells. p40 is equivalent to p63 in sensitivity for squamous cell carcinoma, but it is markedly superior to p63 in specificity, which eliminates a potential pitfall of misinterpreting a p63-positive adenocarcinoma or unsuspected lymphoma as squamous cell carcinoma. These findings strongly support the routine use of p40 in place of p63 for the diagnosis of pulmonary squamous cell carcinoma.
  • Database links

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue labeling p40 - DeltaNp63 with ab167612 at 1/100 dilution.

References

This product has been referenced in:
  • Gu H  et al. Highly expressed histone deacetylase 5 promotes the growth of hepatocellular carcinoma cells by inhibiting the TAp63-maspin pathway. Am J Cancer Res 8:462-475 (2018). WB ; Human . Read more (PubMed: 29637001) »
  • Fu Y  et al. Ductal activation of oncogenic KRAS alone induces sarcomatoid phenotype. Sci Rep 5:13347 (2015). Read more (PubMed: 26289340) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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