This product was changed from ascites to tissue culture supernatant on 20/07/17. The following lots are from ascites and are still in stock as of [20/07/17] – [GR311007, GR322490]. Lot numbers higher than [GR322490] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
pH: 6.00 Preservative: 0.09% Sodium azide Constituent: bovine serum albumin
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Differentiating between lung adenocarcinoma and squamous cell carcinoma is an important distinction with therapeutic implications. Although the most frequently recommended squamous marker p63 is extremely sensitive, it suffers from low specificity due to its reactivity in a substantial proportion of lung adenocarcinomas and other tumor types, particularly lymphomas. p40 is a relatively unknown marker that recognizes ?Np63-a p63 isoform suggested to be highly specific for squamous/basal cells. p40 is equivalent to p63 in sensitivity for squamous cell carcinoma, but it is markedly superior to p63 in specificity, which eliminates a potential pitfall of misinterpreting a p63-positive adenocarcinoma or unsuspected lymphoma as squamous cell carcinoma. These findings strongly support the routine use of p40 in place of p63 for the diagnosis of pulmonary squamous cell carcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lung squamous cell carcinoma labelling p40 - DeltaNp63 with ab172731 for 30 minutes at room temperature. Counterstained with hematoxylin.