Key features and details
- Rabbit polyclonal to p40-phox
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-p40-phox antibody
See all p40-phox primary antibodies
DescriptionRabbit polyclonal to p40-phox
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Recombinant fragment (His-T7-tag) corresponding to Mouse p40-phox aa 111-339. (Expressed in E.coli)
AYMKNLLSLPVCVLMDPDVRIFFYQSAYDAEQVPQALRRLRPRTRKIKGV SPQGAIMDRMEAPRAEALFDFTGNSKLELSFKAGDVIFLLSKINKDWLEG TSQGATGIFPGSFVKILKDFPEDEDTTNWLRCYFYEDTGKTIKDIAVEED LSSTPLFKDLLALMRREFQREDIALSYQDAEGDLVRLLSDEDVGLMVKQA RGLPSQKRLFPWKLHVTQKDNYSVYNTVP
Database link: P97369
- IHC-P: Mouse liver, spleen and kidney tissues. WB: Recombinant mouse p40-phox protein; Mouse spleen lysate.
Previously labelled as NCF4.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231639 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 39 kDa.|
FunctionComponent of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
Tissue specificityExpression is restricted to hematopoietic cells.
Sequence similaritiesContains 1 PX (phox homology) domain.
Contains 1 SH3 domain.
- Information by UniProt
- CGD3 antibody
- MGC3810 antibody
- NCF 4 antibody
Anti-p40-phox antibody (ab231639) at 3 µg/ml + Mouse spleen lysate
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 39 kDa
Anti-p40-phox antibody (ab231639) at 3 µg/ml + Recombinant mouse p40-phox protein.
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 39 kDa
Paraffin-embedded mouse kidney tissue stained for p40-phox using ab231639 at 20 µg/ml in immunohistochemical analysis.
Paraffin-embedded mouse spleen tissue stained for p40-phox using ab231639 at 20 µg/ml in immunohistochemical analysis.
Paraffin-embedded mouse liver tissue stained for p40-phox using ab231639 at 20 µg/ml in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231639 has not yet been referenced specifically in any publications.