Anti-p400 antibody (ab251710)
Key features and details
- Rabbit polyclonal to p400
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p400 antibody
See all p400 primary antibodies -
Description
Rabbit polyclonal to p400 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human p400 aa 900-1100.
Database link: Q96L91 -
Positive control
- IHC-P: Human cerebral cortex, skin and testis tissue. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HSA domain.
Contains 1 Myb-like domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 57634 Human
- Omim: 606265 Human
- SwissProt: Q96L91 Human
- Unigene: 595201 Human
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Alternative names
- CAG repeat protein 32 antibody
- CAGH 32 antibody
- CAGH32 antibody
see all
Images
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Paraffin-embedded human skin tissue stained for p400 using ab251710 at 1/50 dilution in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for p400 (green) using ab251710 at 4 µg/ml in ICC/IF.
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Paraffin-embedded human testis tissue stained for p400 using ab251710 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human cerebral cortex tissue stained for p400 using ab251710 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human liver tissue stained for p400 using ab251710 at 1/50 dilution in immunohistochemical analysis. No nuclear positivity in hepatocytes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab251710 has not yet been referenced specifically in any publications.