Key features and details
- Rabbit polyclonal to p46
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-p46 antibody
See all p46 primary antibodies
DescriptionRabbit polyclonal to p46
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human testis tissue; RT4 and U251 MG cell lysates; A431 cells.
Previously labelled as SKAR.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionIs involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein.
- Information by UniProt
- 46 kDa DNA polymerase delta interaction protein antibody
- DNA polymerase delta interacting protein 3 antibody
- KIAA1649 antibody
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
ab121568 at 1/20 dilution staining p46 in Paraffin-embedded Human Testis tissue by Immunohistochemistry.
ab121568 at 4 µg/ml staining p46 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
All lanes : Anti-p46 antibody (ab121568) at 1/250 dilution
Lane 1 : RT4 lysate
Lane 2 : Human liver lysate
Lane 3 : U251 MG lysate
Lane 4 : Human plasma
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
ab121568 has not yet been referenced specifically in any publications.