Product nameAnti-p46 antibody
See all p46 primary antibodies
DescriptionRabbit polyclonal to p46
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to Human p46 aa 371-421.
Database link: NP_115687.2
- HeLa, 293T and Jurkat whole cell lysate (ab7899)
Previously labelled as SKAR.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab134556 is affinity purified using an epitope specific to p46 immobilized on a solid support.
Our Abpromise guarantee covers the use of ab134556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 46 kDa.|
|IP||Use a concentration of 2 - 10 µg/ml.|
FunctionIs involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein.
- Information by UniProt
- 46 kDa DNA polymerase delta interaction protein antibody
- DNA polymerase delta interacting protein 3 antibody
- KIAA1649 antibody
All lanes : Anti-p46 antibody (ab134556) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 46 kDa
Exposure time: 3 minutes
Detection of p46 by Western blot of immunprecipitate.
Lane 1: ab134556, at a 1 µg/ml, staining p46 in HeLa whole cell lysate immunoprecipitated using ab134556 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: The lysate is precipitated with an IgG control antibody.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab134556 has not yet been referenced specifically in any publications.