Overview

  • Product name

  • Description

    Rabbit polyclonal to p46
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human p46 aa 371-421.
    Database link: NP_115687.2

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysate (ab7899)
  • General notes

    Previously labelled as SKAR. 

Properties

Applications

Our Abpromise guarantee covers the use of ab134556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 46 kDa.
IP Use a concentration of 2 - 10 µg/ml.

Target

  • Function

    Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein.
  • Information by UniProt
  • Database links

  • Alternative names

    • 46 kDa DNA polymerase delta interaction protein antibody
    • DNA polymerase delta interacting protein 3 antibody
    • KIAA1649 antibody
    • P46 antibody
    • PDIP3_HUMAN antibody
    • PDIP46 antibody
    • POLDIP3 antibody
    • Polymerase (DNA-directed), delta interacting protein 3 antibody
    • Polymerase delta interacting protein 46 antibody
    • Polymerase delta-interacting protein 3 antibody
    • RNA-binding protein P46 antibody
    • S6K1 Aly/REF-like target antibody
    • SKAR antibody
    see all

Images

  • All lanes : Anti-p46 antibody (ab134556) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 46 kDa


    Exposure time: 3 minutes
  • Detection of p46 by Western blot of immunprecipitate.
    Lane 1: ab134556, at a 1 µg/ml, staining p46 in HeLa whole cell lysate immunoprecipitated using ab134556 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: The lysate is precipitated with an IgG control antibody.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab134556 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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