Anti-P4HA1 antibody (ab127564)
Key features and details
- Rabbit polyclonal to P4HA1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-P4HA1 antibody
See all P4HA1 primary antibodies -
Description
Rabbit polyclonal to P4HA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 318-517 of Human P4HA1 (P13674).
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Positive control
- PC3 whole cell lysate; Human Hepatoma tissue.
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General notes
Store undiluted.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab127564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 61 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively use Tris-EDTA buffer (pH8.0) |
Target
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Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. -
Sequence similarities
Belongs to the P4HA family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 TPR repeat. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 5033 Human
- SwissProt: P13674 Human
- Unigene: 500047 Human
- Unigene: 593005 Human
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Alternative names
- 2-oxoglutarate-4-dioxygenase subunit alpha-1 antibody
- 4-hydroxylase, alpha I subunit antibody
- 4-PH alpha-1 antibody
see all
Images
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Western blot - Anti-P4HA1 antibody (ab127564)Anti-P4HA1 antibody (ab127564) at 1/1000 dilution + PC3 whole cell lysate at 30 µg
Predicted band size: 61 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (ab127564)ab127564 at 1/500 dilution staining P4HA1 in paraffin-embedded Human Hepatoma tissue by Immunohistochemistry.
Protocols
References (0)
ab127564 has not yet been referenced specifically in any publications.
