Anti-P4HA1 antibody (ab127564)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-P4HA1 antibody
See all P4HA1 primary antibodies -
Description
Rabbit polyclonal to P4HA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 318-517 of Human P4HA1 (P13674).
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Positive control
- PC3 whole cell lysate; Human Hepatoma tissue.
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General notes
Store undiluted.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab127564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 61 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively use Tris-EDTA buffer (pH8.0) |
Target
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Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. -
Sequence similarities
Belongs to the P4HA family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 TPR repeat. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 5033 Human
- SwissProt: P13674 Human
- Unigene: 500047 Human
- Unigene: 593005 Human
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Alternative names
- 2-oxoglutarate-4-dioxygenase subunit alpha-1 antibody
- 4-hydroxylase, alpha I subunit antibody
- 4-PH alpha-1 antibody
see all
Images
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Western blot - Anti-P4HA1 antibody (ab127564)Anti-P4HA1 antibody (ab127564) at 1/1000 dilution + PC3 whole cell lysate at 30 µg
Predicted band size: 61 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA1 antibody (ab127564)ab127564 at 1/500 dilution staining P4HA1 in paraffin-embedded Human Hepatoma tissue by Immunohistochemistry.
References
ab127564 has not yet been referenced specifically in any publications.
