Anti-P4HA1 antibody (ab244400)
Key features and details
- Rabbit polyclonal to P4HA1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-P4HA1 antibody
See all P4HA1 primary antibodies -
Description
Rabbit polyclonal to P4HA1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan -
Immunogen
Recombinant fragment within Human P4HA1 aa 150-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P13674 -
Positive control
- ICC/IF: U-2 OS cells. WB: PC-3 and HEK293T cell lysate. NIH3T3 and NBT-II cell lysate. IHC-P: Human placenta, endometrium, testis, cerebellum and pancreas tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. -
Sequence similarities
Belongs to the P4HA family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 TPR repeat. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 5033 Human
- Entrez Gene: 18451 Mouse
- Entrez Gene: 64475 Rat
- SwissProt: P13674 Human
- SwissProt: Q60715 Mouse
- SwissProt: P54001 Rat
- Unigene: 500047 Human
- Unigene: 593005 Human
see all -
Alternative names
- 2-oxoglutarate-4-dioxygenase subunit alpha-1 antibody
- 4-hydroxylase, alpha I subunit antibody
- 4-PH alpha-1 antibody
see all
Images
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Paraffin-embedded human placenta tissue stained for P4HA1 using ab244400 at 1/2500 dilution in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for P4HA1 (green) using ab244400 at 4 µg/ml in ICC/IF.
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All lanes : Anti-P4HA1 antibody (ab244400) at 0.4 µg/ml
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (Rat cell line) cell lysate -
All lanes : Anti-P4HA1 antibody (ab244400) at 0.4 µg/ml
Lane 1 : PC-3 (Human prostate adenocarcinoma cell line) cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate -
Paraffin-embedded human endometrium tissue stained for P4HA1 using ab244400 at 1/2500 dilution in immunohistochemical analysis.
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Paraffin-embedded human testis tissue stained for P4HA1 using ab244400 at 1/2500 dilution in immunohistochemical analysis.
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Paraffin-embedded human cerebellum tissue stained for P4HA1 using ab244400 at 1/2500 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreas tissue stained for P4HA1 using ab244400 at 1/2500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab244400 has been referenced in 2 publications.
- Li Y et al. The Role of P4HA1 in Multiple Cancer Types and its Potential as a Target in Renal Cell Carcinoma. Front Genet 13:848456 (2022). PubMed: 35812752
- Cao XP et al. P4HA1/HIF1a feedback loop drives the glycolytic and malignant phenotypes of pancreatic cancer. Biochem Biophys Res Commun 516:606-612 (2019). PubMed: 31239153