Overview

  • Product name

  • Description

    Mouse polyclonal to P4HA1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length Human P4HA1 (NP_001017962.1)

  • Positive control

    • A431 whole cell lysate (ab7909) can be used as a positive control in WB. A-431 cell lysate and P4HA1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab88881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa.

Target

  • Function

    Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
  • Sequence similarities

    Belongs to the P4HA family.
    Contains 1 Fe2OG dioxygenase domain.
    Contains 1 TPR repeat.
  • Cellular localization

    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2-oxoglutarate-4-dioxygenase subunit alpha-1 antibody
    • 4-hydroxylase, alpha I subunit antibody
    • 4-PH alpha-1 antibody
    • P4HA antibody
    • P4HA1 antibody
    • P4HA1_HUMAN antibody
    • Procollagen-proline antibody
    • Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I antibody
    • Prolyl 4-hydroxylase subunit alpha-1 antibody
    see all

Images

  • Anti-P4HA1 antibody (ab88881) at 1 µg/ml + A-431 cell lysate at 50 µg

    Predicted band size: 61 kDa
    Observed band size: 61 kDa

  • All lanes : Anti-P4HA1 antibody (ab88881) at 1 µg/ml

    Lane 1 : P4HA1 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 61 kDa
    Observed band size: 61 kDa

References

This product has been referenced in:

  • Dai L  et al. Differential profiling studies of N-linked glycoproteins in glioblastoma cancer stem cells upon treatment with ?-secretase inhibitor. Proteomics 11:4021-8 (2011). Read more (PubMed: 21898824) »
See 1 Publication for this product

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