Key features and details
- Rabbit polyclonal to P4HA2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-P4HA2 antibody
See all P4HA2 primary antibodies
DescriptionRabbit polyclonal to P4HA2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment (His-T7-tag) corresponding to Human P4HA2 aa 207-520. Two N-terminal tags. (Expressed in E.coli).
SQVLDYLSYAVFQLGDLHRALELTRRLLSLDPSHERAGGNLRYFEQLLEE EREKTLTNQTEAELATPEGIYERPVDYLPERDVYESLCRGEGVKLTPRRQ KRLFCRYHHGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEI AKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVARVNRRMQHIT GLTVKTAELLQVANYGVGGQYEPHFDFSRNDERDTFKHLGTGNRVATFLN YMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPV LVGCKWVSNKWFHE
Database link: O15460
- ICC/IF: HeLa cells. IHC-P: Human pancreas tissue. WB: Recombinant human P4HA2 protein; HeLa and HEK-293T cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
FunctionCatalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Sequence similaritiesBelongs to the P4HA family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 TPR repeat.
Cellular localizationEndoplasmic reticulum lumen.
- Information by UniProt
- 2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody
- 4 PH alpha 2 antibody
- 4-PH alpha-2 antibody
All lanes : Anti-P4HA2 antibody (ab233197) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Formalin-fixed, paraffin-embedded human pancreas tissue stained for P4HA2 using ab233197 at 20 µg/ml in immunohistochemical analysis. DAB staining.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for P4HA2 using ab233197 at 2 µg/ml in ICC/IF. FITC staining.
Anti-P4HA2 antibody (ab233197) at 2 µg/ml + Recombinant human P4HA2 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233197 has not yet been referenced specifically in any publications.