Product nameAnti-P4HB antibody
See all P4HB primary antibodies
DescriptionRabbit polyclonal to P4HB
SpecificityP4HB (PDIA1) runs at 61.2 kD on samples from human placenta. In samples from rat liver, it runs at 59 kD, but 3 other bands may appear due to protein degradation - these run at 58.9 kD, 55.2 kD and 53.8 kD.
Tested applicationsSuitable for: ICC, WB, IHC-P, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Hamster, Cow, Human, African green monkey, Chinese hamster
Full length protein corresponding to P4HB.
Database link: P07237
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab3672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/50 - 1/100.|
|WB||1/1000. Detects a band of approximately 60 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/600. PubMed: 19059915|
FunctionThis multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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ICC/IF image of ab3672 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3672, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
Anti-P4HB antibody (ab3672) at 1/1000 dilution + PC12 whole cell lysate at 20 µg
HRP conjugated swine anti-rabbit antibody
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa, 48 kDa, 58 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab3672 at 1/400 staining mouse kidney tissue sections by IHC-P. The tissue was formladehyde fixed and a heat mediated antigen retrieval step was performed in citrate buffer. The tissue was incubated with the primary antibody for 30 minutes at 22°C and then an HRP conjugated goat anti-rabbit antibody was used as the secondary.
Chinese Hamster Ovary cells stained for P4HB (PDIA1) using ab3672 at 1/200 dilution ICC/IF. Cy3®-labeled secondary antibody.
ab3672 staining mouse cortical oligodendrocytes from vibratome sections by IHC-Fr. Sections were fixed in Somogyi (PFA + glutaraldehyde + picric acid) or PLP (Periodate-lysine-PFA) and permeabilized in TX-100, prior to blocking with 2.5% serum for 2 hours at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 18 hours at 25°C. A TRITC conjugated pig anti-rabbit IgG antibody, diluted 1/1000, was used as the secondary.
ab3672 staining P4HB (PDIA1) in human HaCaT Keratinocyte by ICC/IF. The cells were paraformaldehyde fixed, permeabilized in 0.25% triton X-100 buffer and blocked in 2.5% BSA plus 1% goat serum for 1 hour at 4°C. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 24°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary.
This product has been referenced in:
- Simonin Y et al. Zika virus induces strong inflammatory responses and impairs homeostasis and function of the human retinal pigment epithelium. EBioMedicine 39:315-331 (2019). Read more (PubMed: 30579862) »
- Wang H et al. N-glycosylation in the protease domain of trypsin-like serine proteases mediates calnexin-assisted protein folding. Elife 7:N/A (2018). Read more (PubMed: 29889025) »