Immunocytochemistry/ Immunofluorescence abreview for Anti-P4HB antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HaCaT keratinocyte)
Permeabilization
Yes - 0.25% triton X-100
Specification
HaCaT keratinocyte
Blocking step
2.5% BSA plus 1% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: TBST plus 1/10 block
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: goat anti-rabbit (invitrogen)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
punctate staining seen throughout cell cytoplasm, presumably ER. Certain regions stained brighter than others. Nuclei stained with DAPI

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Submitted Jun 05 2009

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