Immunocytochemistry/ Immunofluorescence abreview for Anti-P4HB antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Chinese hamster Cell (ovary)
Permeabilization
Yes - 0.1% Triton X-100
Specification
ovary
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Methanol

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/200

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti-rabbit F(ab')2 fragment
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®

Additional data

Additional Notes
Blue = DAPI

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Submitted Oct 08 2010

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