Immunocytochemistry/ Immunofluorescence abreview for Anti-P4HB antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
No
Specification
HEK293
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: Donkey serum in PBST
Dilution
1/100

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Donkey-anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: DyLight® 594

Additional data

Additional Notes
Red - PDI Green - Golgi (stained by C2GnT-M-GFP-Golgi residential enzyme tagged with GFP) Blue- nucles stained by DAPI

Dr. Armen Petrosyan

Verified customer

Submitted Dec 03 2012

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