Western blot abreview for Anti-P4HB antibody

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Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
PC12
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Milk, 0.1% Tween-20, PBS
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Swine anti rabbit-HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 60 kDa Non-specific: 75, 58, 48, 36 kDa

Additional data

Additional Notes
This antibody works well in western blotting- detecting a dominant species of the predicted MW. However, a number of cross-reacting species are observed - although some of these may be degradation products.

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Submitted Jul 13 2007

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