Western blot abreview for Anti-P4HB antibody

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Abreviews
Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Cos-7 cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Cos-7 cell line
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Milk 0.1% Tween-20 in PBS
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Swine anti-rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: ~60 kDa Non-specific: ~65, ~32 kDa

Additional data

Additional Notes
Asterisk indicates putative full-length PDI in Cos-7 cells

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Submitted Sep 20 2007

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