Immunohistochemistry (Frozen sections) abreview for Anti-P4HB antibody

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Cortical oligodendrocytes in vibratome sections)
Permeabilization
Yes - TX100
Specification
Cortical oligodendrocytes in vibratome sections
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Somogy's. PFA + picric acid PLP also, PFA + lysine + periodate

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS, 2.5% serum, 0.5% TX100
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Pig anti rabbit immunoglobulins
Host species: Pig
Clonality: Polyclonal
Conjugation: TRITC

Dr. Guillermo Estivill-Torrus

Verified customer

Submitted Oct 31 2007

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