Anti-P4HB antibody (ab70415)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-P4HB antibody
See all P4HB primary antibodies -
Description
Mouse polyclonal to P4HB -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length human P4HB protein (NP_000909.2)
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Positive control
- Human liver tissue lysate and P4HB transfected 293T lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab70415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
Target
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Function
This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. -
Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 5034 Human
- Omim: 176790 Human
- SwissProt: P07237 Human
- Unigene: 464336 Human
- Unigene: 708779 Human
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Alternative names
- Cellular thyroid hormone binding protein antibody
- Cellular thyroid hormone-binding protein antibody
- Collagen prolyl 4 hydroxylase beta antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody (ab70415)ab70415 at 10µg/ml staining P4HB in Hela cells by Immunocytochemistry/Immunofluorescence.
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Western blot - Anti-P4HB antibody (ab70415)Anti-P4HB antibody (ab70415) at 1/500 dilution + Human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa -
Western blot - Anti-P4HB antibody (ab70415)All lanes : Anti-P4HB antibody (ab70415) at 1/500 dilution
Lane 1 : P4HB transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 57 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody (ab70415)IHC image of ab70415 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70415, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Gharesi-Fard B et al. Proteome Differences in the First- and Third-Trimester Human Placentas. Reprod Sci : (2014). Read more (PubMed: 25201741) »
