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    p4hb-antibody-ab70415.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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Anti-P4HB antibody (ab70415)

  • Datasheet
Submit a review Submit a question References (1)

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Abpromise

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Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody (ab70415)
  • Western blot - Anti-P4HB antibody (ab70415)
  • Western blot - Anti-P4HB antibody (ab70415)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody (ab70415)

Key features and details

  • Mouse polyclonal to P4HB
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-P4HB antibody
    See all P4HB primary antibodies
  • Description

    Mouse polyclonal to P4HB
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length human P4HB protein (NP_000909.2)

  • Positive control

    • Human liver tissue lysate and P4HB transfected 293T lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)

Applications

Our Abpromise guarantee covers the use of ab70415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

Target

  • Function

    This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P07237 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5034 Human
    • Omim: 176790 Human
    • SwissProt: P07237 Human
    • Unigene: 464336 Human
    • Unigene: 708779 Human
    • Alternative names

      • Cellular thyroid hormone binding protein antibody
      • Cellular thyroid hormone-binding protein antibody
      • Collagen prolyl 4 hydroxylase beta antibody
      • Disulphide Isomerase antibody
      • DSI antibody
      • EC 5.3.4.1 antibody
      • Endoplasmic reticulum resident protein 59 antibody
      • ER protein 59 antibody
      • ERBA2L antibody
      • ERp59 antibody
      • GIT antibody
      • Gltathione insulin transhydrogenase antibody
      • Glutathione insulin transhydrogenase antibody
      • P4HB antibody
      • P4Hbeta antibody
      • p55 antibody
      • PDI antibody
      • PDIA1 antibody
      • PDIA1_HUMAN antibody
      • PDIR antibody
      • PHDB antibody
      • PO4DB antibody
      • PO4HB antibody
      • Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1) antibody
      • Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit antibody
      • PROHB antibody
      • Prolyl 4 hydroxylase beta polypeptide antibody
      • Prolyl 4 hydroxylase beta subunit antibody
      • Prolyl 4 hydroxylase subunit beta antibody
      • Prolyl 4-hydroxylase subunit beta antibody
      • Protein disulfide isomerase associated 1 antibody
      • Protein disulfide isomerase, family A, member 1 antibody
      • Protein disulfide isomerase/oxidoreductase antibody
      • Protein disulfide-isomerase antibody
      • Protocollagen hydroxylase antibody
      • Thbp antibody
      • Thyroid hormone binding protein p55 antibody
      • Thyroid hormone binding protein p55 cellular antibody
      • V erb a avian erythroblastic leukemia viral oncogene homolog 2 like antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody (ab70415)
      Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody (ab70415)

      ab70415 at 10µg/ml staining P4HB in Hela cells by Immunocytochemistry/Immunofluorescence.

    • Western blot - Anti-P4HB antibody (ab70415)
      Western blot - Anti-P4HB antibody (ab70415)
      Anti-P4HB antibody (ab70415) at 1/500 dilution + Human liver tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size: 57 kDa
      Observed band size: 57 kDa

    • Western blot - Anti-P4HB antibody (ab70415)
      Western blot - Anti-P4HB antibody (ab70415)
      All lanes : Anti-P4HB antibody (ab70415) at 1/500 dilution

      Lane 1 : P4HB transfected 293T lysate
      Lane 2 : Non-transfected 293T lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size: 57 kDa
      Observed band size: 62 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody (ab70415)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody (ab70415)
      IHC image of ab70415 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70415, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab70415? Please let us know so that we can cite the reference in this datasheet.

    ab70415 has been referenced in 1 publication.

    • Gharesi-Fard B  et al. Proteome Differences in the First- and Third-Trimester Human Placentas. Reprod Sci : (2014). PubMed: 25201741

    Customer reviews and Q&As

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