Key features and details
- Rabbit polyclonal to P4HB
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-P4HB antibody
See all P4HB primary antibodies
DescriptionRabbit polyclonal to P4HB
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow
Recombinant fragment corresponding to a region within amino acids 73-197 of Human P4HB (NP_000909).
- 293T, A431 and H1299 whole cell lysates; A431 cells; HeLa S3, HepG2, MOLT4 and Raji cell lines
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab96744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 57 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionThis multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Cellular thyroid hormone binding protein antibody
- Cellular thyroid hormone-binding protein antibody
- Collagen prolyl 4 hydroxylase beta antibody
All lanes : Anti-P4HB antibody (ab96744) at 1/500 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
7.5% SDS PAGE
ab96744, at 1/200 dilution, staining P4HB in methanol-fixed A431 cells by Immunofluorescence analysis. Right image is costained with Hoechst 33342.
ab96744 has not yet been referenced specifically in any publications.