Key features and details
- Rabbit polyclonal to P4HB - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-P4HB antibody - C-terminal
See all P4HB primary antibodies
DescriptionRabbit polyclonal to P4HB - C-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Rat P4HB aa 409-509 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P04785
- WB: Rat brain, heart, kidney, liver, lung, pancreas, skeletal muscle, spleen, testis and thymus lysates; C2C12, NIH/3T3 and Pam212 cell lysates. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 57 kDa).|
FunctionThis multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Cellular thyroid hormone binding protein antibody
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2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for P4HB (yellow) using ab223612 at 1/100 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit R-PE at 1/200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at room temperature.
(A) DAPI (blue) nuclear stain (B) ab223612 (C) Merge.
All lanes : Anti-P4HB antibody - C-terminal (ab223612) at 1/4000 dilution
Lane 1 : P4HB purified protein at 0.05 µg
Lane 2 : Rat brain lysate at 15 µg
Lane 3 : Rat heart lysate at 15 µg
Lane 4 : Rat kidney lysate at 15 µg
Lane 5 : Rat liver lysate at 15 µg
Lane 6 : Rat lung lysate at 15 µg
Lane 7 : Rat pancreas lysate at 15 µg
Lane 8 : Rat skeletal muscle lysate at 15 µg
Lane 9 : Rat spleen lysate at 15 µg
Lane 10 : Rat testis lysate at 15 µg
Lane 11 : Rat thymus lysate at 15 µg
Lane 12 : C2C12 (mouse myoblast cell line) cell lysate at 15 µg
Lane 13 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate at 15 µg
Lane 14 : Pam212 cell lysate at 15 µg
All lanes : Donkey Anti-Rabbit IgG HRP
Predicted band size: 57 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for P4HB (green) using ab223612 at 1/100 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit FITC at 1/200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at room temperature.
(A) DAPI (blue) nuclear stain (B) ab223612 (C) Merge.
ab223612 has not yet been referenced specifically in any publications.