Overview

  • Product name

    Anti-P4HB antibody - C-terminal
    See all P4HB primary antibodies
  • Description

    Rabbit polyclonal to P4HB - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Rat P4HB aa 409-509 (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P04785

  • Positive control

    • WB: Rat brain, heart, kidney, liver, lung, pancreas, skeletal muscle, spleen, testis and thymus lysates; C2C12, NIH/3T3 and Pam212 cell lysates. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 57 kDa).
ICC/IF 1/100.

Target

  • Function

    This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cellular thyroid hormone binding protein antibody
    • Cellular thyroid hormone-binding protein antibody
    • Collagen prolyl 4 hydroxylase beta antibody
    • Disulphide Isomerase antibody
    • DSI antibody
    • EC 5.3.4.1 antibody
    • Endoplasmic reticulum resident protein 59 antibody
    • ER protein 59 antibody
    • ERBA2L antibody
    • ERp59 antibody
    • GIT antibody
    • Gltathione insulin transhydrogenase antibody
    • Glutathione insulin transhydrogenase antibody
    • P4HB antibody
    • P4Hbeta antibody
    • p55 antibody
    • PDI antibody
    • PDIA1 antibody
    • PDIA1_HUMAN antibody
    • PDIR antibody
    • PHDB antibody
    • PO4DB antibody
    • PO4HB antibody
    • Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1) antibody
    • Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit antibody
    • PROHB antibody
    • Prolyl 4 hydroxylase beta polypeptide antibody
    • Prolyl 4 hydroxylase beta subunit antibody
    • Prolyl 4 hydroxylase subunit beta antibody
    • Prolyl 4-hydroxylase subunit beta antibody
    • Protein disulfide isomerase associated 1 antibody
    • Protein disulfide isomerase, family A, member 1 antibody
    • Protein disulfide isomerase/oxidoreductase antibody
    • Protein disulfide-isomerase antibody
    • Protocollagen hydroxylase antibody
    • Thbp antibody
    • Thyroid hormone binding protein p55 antibody
    • Thyroid hormone binding protein p55 cellular antibody
    • V erb a avian erythroblastic leukemia viral oncogene homolog 2 like antibody
    see all

Images

  • 2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for P4HB (yellow) using ab223612 at 1/100 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit R-PE at 1/200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at room temperature.

    (A) DAPI (blue) nuclear stain (B) ab223612 (C) Merge.

  • All lanes : Anti-P4HB antibody - C-terminal (ab223612) at 1/4000 dilution

    Lane 1 : P4HB purified protein at 0.05 µg
    Lane 2 : Rat brain lysate at 15 µg
    Lane 3 : Rat heart lysate at 15 µg
    Lane 4 : Rat kidney lysate at 15 µg
    Lane 5 : Rat liver lysate at 15 µg
    Lane 6 : Rat lung lysate at 15 µg
    Lane 7 : Rat pancreas lysate at 15 µg
    Lane 8 : Rat skeletal muscle lysate at 15 µg
    Lane 9 : Rat spleen lysate at 15 µg
    Lane 10 : Rat testis lysate at 15 µg
    Lane 11 : Rat thymus lysate at 15 µg
    Lane 12 : C2C12 (mouse myoblast cell line) cell lysate at 15 µg
    Lane 13 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate at 15 µg
    Lane 14 : Pam212 cell lysate at 15 µg

    Secondary
    All lanes : Donkey Anti-Rabbit IgG HRP

    Predicted band size: 57 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?

  • 2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for P4HB (green) using ab223612 at 1/100 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit FITC at 1/200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at room temperature.

    (A) DAPI (blue) nuclear stain (B) ab223612 (C) Merge.

References

ab223612 has not yet been referenced specifically in any publications.

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