Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-P4HB antibody [EPR9498] (ab137119)

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Overview

  • Product name

    Anti-P4HB antibody [EPR9498]
    See all P4HB primary antibodies

  • Description

    Rabbit monoclonal [EPR9498] to P4HB

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: FM,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human P4HB aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human P4HB protein (ab115719) can be used as a positive control in WB. HeLa, HepG2, A431, and MCF7 cell lysates, Human colon adenocarcinoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab137119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for FM,ICC/IF or IP.

    • Target

    • Function

      This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.

    • Sequence similarities

      Belongs to the protein disulfide isomerase family.
      Contains 2 thioredoxin domains.

    • Cellular localization

      Endoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Cellular thyroid hormone binding protein antibody
      • Cellular thyroid hormone-binding protein antibody
      • Collagen prolyl 4 hydroxylase beta antibody
      • Disulphide Isomerase antibody
      • DSI antibody
      • EC 5.3.4.1 antibody
      • Endoplasmic reticulum resident protein 59 antibody
      • ER protein 59 antibody
      • ERBA2L antibody
      • ERp59 antibody
      • GIT antibody
      • Gltathione insulin transhydrogenase antibody
      • Glutathione insulin transhydrogenase antibody
      • P4HB antibody
      • P4Hbeta antibody
      • p55 antibody
      • PDI antibody
      • PDIA1 antibody
      • PDIA1_HUMAN antibody
      • PDIR antibody
      • PHDB antibody
      • PO4DB antibody
      • PO4HB antibody
      • Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1) antibody
      • Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit antibody
      • PROHB antibody
      • Prolyl 4 hydroxylase beta polypeptide antibody
      • Prolyl 4 hydroxylase beta subunit antibody
      • Prolyl 4 hydroxylase subunit beta antibody
      • Prolyl 4-hydroxylase subunit beta antibody
      • Protein disulfide isomerase associated 1 antibody
      • Protein disulfide isomerase, family A, member 1 antibody
      • Protein disulfide isomerase/oxidoreductase antibody
      • Protein disulfide-isomerase antibody
      • Protocollagen hydroxylase antibody
      • Thbp antibody
      • Thyroid hormone binding protein p55 antibody
      • Thyroid hormone binding protein p55 cellular antibody
      • V erb a avian erythroblastic leukemia viral oncogene homolog 2 like antibody
      see all

    Images

    • Western blot - Anti-P4HB antibody [EPR9498] (ab137119)
      Western blot - Anti-P4HB antibody [EPR9498] (ab137119)
      All lanes : Anti-P4HB antibody [EPR9498] (ab137119) at 1 µg/ml

      Lane 1 : Wild-type HeLa whole cell lysate
      Lane 2 : P4HB knockout HeLa whole cell lysate
      Lane 3 : HEP G2 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 57 kDa



      Lanes 1 - 3: Merged signal (red and green). Green - ab137119 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab137119 was shown to specifically react with P4HB (Protein disulfide-isomerase) in wild-type HeLa cells as signal was lost in P4HB knockout cells. Wild-type and P4HB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab137119 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-P4HB antibody [EPR9498] (ab137119)
      Western blot - Anti-P4HB antibody [EPR9498] (ab137119)
      All lanes : Anti-P4HB antibody [EPR9498] (ab137119) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 57 kDa
      Observed band size: 57 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [EPR9498] (ab137119)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [EPR9498] (ab137119)

      Immunohistochemical analysis of paraffin embedded Human colon adenocarcinoma tissue labelling P4HB with ab137119 antibody at a dilution of 1/50.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Sahebekhtiari N  et al. Deficiency of the mitochondrial sulfide regulator ETHE1 disturbs cell growth, glutathione level and causes proteome alterations outside mitochondria. Biochim Biophys Acta Mol Basis Dis 1865:126-135 (2019). Read more (PubMed: 30391543) »
    See 1 Publication for this product

    Publishing research using ab137119? Please let us know so that we can cite the reference in this datasheet.

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