Overview

  • Product name

    Anti-p50 dynamitin antibody
    See all p50 dynamitin primary antibodies
  • Description

    Rabbit polyclonal to p50 dynamitin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Chimpanzee
  • Immunogen

    Recombinant fragment within Human p50 dynamitin (internal sequence). The exact sequence is proprietary.
    Database link: Q13561

  • Positive control

    • WB: MCF7 whole cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: MCF7 cells.
  • General notes

    Previously labelled as Dynamitin. 

Properties

Applications

Our Abpromise guarantee covers the use of ab228704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
  • Sequence similarities

    Belongs to the dynactin subunit 2 family.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 50 kD dynein associated polypeptide antibody
    • 50 kDa dynein associated polypeptide antibody
    • 50 kDa dynein-associated polypeptide antibody
    • DCTN-50 antibody
    • DCTN2 antibody
    • DCTN2_HUMAN antibody
    • DCTN50 antibody
    • dynactin 2 (p50) antibody
    • dynactin 2 antibody
    • dynactin complex 50 kD subunit antibody
    • Dynactin complex 50 kDa subunit antibody
    • Dynactin subunit 2 antibody
    • DYNAMITIN antibody
    • p50 dynamitin antibody
    • RBP50 antibody
    see all

Images

  • Anti-p50 dynamitin antibody (ab228704) at 1/3000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 44 kDa



    10% SDS-PAGE

  • Methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for p50 dynamitin (green) using ab228704 at a 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Paraffin-embedded human breast cancer tissue stained for p50 dynamitin with ab228704  at 1/250 dilution in immunohistochemical analysis.

References

ab228704 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228704.
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