Recombinant Anti-p50 dynamitin antibody [EPR5095] (ab133492)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5095] to p50 dynamitin
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-p50 dynamitin antibody [EPR5095]
See all p50 dynamitin primary antibodies -
Description
Rabbit monoclonal [EPR5095] to p50 dynamitin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human p50 dynamitin aa 300-400.
Database link: Q13561 -
Positive control
- WB: MCF7, A375, Saos2, and SH SY5Y cell lysates. ICC/IF: SHSY5Y cells. IHC-P: Human cerebrum tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5095 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
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ICC/IF |
1/100 - 1/250.
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IHC-P |
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). |
ICC/IF
1/100 - 1/250. |
IHC-P
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. -
Sequence similarities
Belongs to the dynactin subunit 2 family. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10540 Human
- Omim: 607376 Human
- SwissProt: Q13561 Human
- Unigene: 289123 Human
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Alternative names
- 50 kD dynein associated polypeptide antibody
- 50 kDa dynein associated polypeptide antibody
- 50 kDa dynein-associated polypeptide antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling p50 dynamitin with ab133492 at 1/400 dilution followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on human cerebrum. The section was incubated with ab133492 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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All lanes : Anti-p50 dynamitin antibody [EPR5095] (ab133492) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : A375 cell lysate
Lane 3 : Saos2 cell lysate
Lane 4 : SH SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa -
Immunofluorescence analysis of p50 dynamitin in SH-SY5Y cells, using ab133492 at a dilution of 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab133492 has been referenced in 6 publications.
- Zhou HJ et al. lncRNA MEG3 restrained the M1 polarization of microglia in acute spinal cord injury through the HuR/A20/NF-κB axis. Brain Pathol 32:e13070 (2022). PubMed: 35338543
- Chang J et al. Glabridin attenuates atopic dermatitis progression through downregulating the TLR4/MyD88/NF-?B signaling pathway. Genes Genomics 43:847-855 (2021). PubMed: 34021857
- Villari G et al. Distinct retrograde microtubule motor sets drive early and late endosome transport. EMBO J 39:e103661 (2020). PubMed: 33215754
- Dwivedi D et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). PubMed: 30674580
- Weisell J et al. Heat shock protein 90 is downregulated in calcific aortic valve disease. BMC Cardiovasc Disord 19:306 (2019). PubMed: 31856737
- Wilson GN et al. Early Cytoskeletal Protein Modifications Precede Overt Structural Degeneration in the DBA/2J Mouse Model of Glaucoma. Front Neurosci 10:494 (2016). IP . PubMed: 27857681