Recombinant Anti-p50 dynamitin antibody [EPR5095] (ab133492)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5095] to p50 dynamitin
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-p50 dynamitin antibody [EPR5095]
See all p50 dynamitin primary antibodies -
Description
Rabbit monoclonal [EPR5095] to p50 dynamitin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human p50 dynamitin aa 300-400.
Database link: Q13561 -
Positive control
- MCF7, A375, Saos2, and SH SY5Y cell lysates; SHSY5Y cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Dynamitin.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5095 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). | |
ICC/IF | 1/100 - 1/250. |
Target
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Function
Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. -
Sequence similarities
Belongs to the dynactin subunit 2 family. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10540 Human
- Omim: 607376 Human
- SwissProt: Q13561 Human
- Unigene: 289123 Human
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Alternative names
- 50 kD dynein associated polypeptide antibody
- 50 kDa dynein associated polypeptide antibody
- 50 kDa dynein-associated polypeptide antibody
see all
Images
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All lanes : Anti-p50 dynamitin antibody [EPR5095] (ab133492) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : A375 cell lysate
Lane 3 : Saos2 cell lysate
Lane 4 : SH SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa -
Immunofluorescence analysis of p50 dynamitin in SH-SY5Y cells, using ab133492 at a dilution of 1/100.
Protocols
Datasheets and documents
References (3)
ab133492 has been referenced in 3 publications.
- Dwivedi D et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). PubMed: 30674580
- Weisell J et al. Heat shock protein 90 is downregulated in calcific aortic valve disease. BMC Cardiovasc Disord 19:306 (2019). PubMed: 31856737
- Wilson GN et al. Early Cytoskeletal Protein Modifications Precede Overt Structural Degeneration in the DBA/2J Mouse Model of Glaucoma. Front Neurosci 10:494 (2016). IP . PubMed: 27857681