Recombinant
RabMAb

Recombinant Anti-p50 dynamitin antibody [EPR5095] (ab133492)

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Overview

  • Product name

    Anti-p50 dynamitin antibody [EPR5095]
    See all p50 dynamitin primary antibodies

  • Description

    Rabbit monoclonal [EPR5095] to p50 dynamitin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human p50 dynamitin aa 300-400.
    Database link: Q13561

  • Positive control

    • MCF7, A375, Saos2, and SH SY5Y cell lysates; SHSY5Y cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as Dynamitin. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.

    • Sequence similarities

      Belongs to the dynactin subunit 2 family.

    • Cellular localization

      Cytoplasm > cytoskeleton > centrosome. Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • 50 kD dynein associated polypeptide antibody
        • 50 kDa dynein associated polypeptide antibody
        • 50 kDa dynein-associated polypeptide antibody
        • DCTN-50 antibody
        • DCTN2 antibody
        • DCTN2_HUMAN antibody
        • DCTN50 antibody
        • dynactin 2 (p50) antibody
        • dynactin 2 antibody
        • dynactin complex 50 kD subunit antibody
        • Dynactin complex 50 kDa subunit antibody
        • Dynactin subunit 2 antibody
        • DYNAMITIN antibody
        • p50 dynamitin antibody
        • RBP50 antibody
        see all

      Images

      • Western blot - Anti-p50 dynamitin antibody [EPR5095] (ab133492)
        Western blot - Anti-p50 dynamitin antibody [EPR5095] (ab133492)
        All lanes : Anti-p50 dynamitin antibody [EPR5095] (ab133492) at 1/1000 dilution

        Lane 1 : MCF7 cell lysate
        Lane 2 : A375 cell lysate
        Lane 3 : Saos2 cell lysate
        Lane 4 : SH SY5Y cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 44 kDa
        Observed band size: 44 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-p50 dynamitin antibody [EPR5095] (ab133492)
        Immunocytochemistry/ Immunofluorescence - Anti-p50 dynamitin antibody [EPR5095] (ab133492)

        Immunofluorescence analysis of p50 dynamitin in SH-SY5Y cells, using ab133492 at a dilution of 1/100.

      References

      This product has been referenced in:

      • Dwivedi D  et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). Read more (PubMed: 30674580) »
      • Wilson GN  et al. Early Cytoskeletal Protein Modifications Precede Overt Structural Degeneration in the DBA/2J Mouse Model of Glaucoma. Front Neurosci 10:494 (2016). IP . Read more (PubMed: 27857681) »
      See all 2 Publications for this product

      Publishing research using ab133492? Please let us know so that we can cite the reference in this datasheet.

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