Product nameAnti-p50 dynamitin antibody [EPR5095]
See all p50 dynamitin primary antibodies
DescriptionRabbit monoclonal [EPR5095] to p50 dynamitin
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human p50 dynamitin aa 300-400.
Database link: Q13561
- MCF7, A375, Saos2, and SH SY5Y cell lysates; SHSY5Y cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Dynamitin.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
FunctionModulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
Sequence similaritiesBelongs to the dynactin subunit 2 family.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Membrane.
- Information by UniProt
- 50 kD dynein associated polypeptide antibody
- 50 kDa dynein associated polypeptide antibody
- 50 kDa dynein-associated polypeptide antibody
All lanes : Anti-p50 dynamitin antibody [EPR5095] (ab133492) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : A375 cell lysate
Lane 3 : Saos2 cell lysate
Lane 4 : SH SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Immunofluorescence analysis of p50 dynamitin in SH-SY5Y cells, using ab133492 at a dilution of 1/100.
This product has been referenced in:
- Dwivedi D et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). Read more (PubMed: 30674580) »
- Wilson GN et al. Early Cytoskeletal Protein Modifications Precede Overt Structural Degeneration in the DBA/2J Mouse Model of Glaucoma. Front Neurosci 10:494 (2016). IP . Read more (PubMed: 27857681) »