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Cell Biology Cell Cycle Cell Cycle Inhibitors p53
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RecombinantRabMAb

Recombinant Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)

  • Datasheet
  • SDS
Reviews (4)Q&A (2)References (29)

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Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
  • Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
  • Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
  • Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
  • Flow Cytometry - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
  • Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR358(2)] to p53 (acetyl K382)
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-p53 (acetyl K382) antibody [EPR358(2)]
    See all p53 primary antibodies
  • Description

    Rabbit monoclonal [EPR358(2)] to p53 (acetyl K382)
  • Host species

    Rabbit
  • Specificity

    ab75754 only detects p53 acetylated on Lysine 382.
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human p53 aa 350 to the C-terminus (acetyl K382). The exact sequence is proprietary.
    Database link: P04637
    (Peptide available as ab183615)

  • Positive control

    • WB: HepG2 cell lysates treated with etopside and TSA. ICC/IF: HeLa cells Flow Cyt: HepG2 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR358(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • p53
    • Cell Biology
    • Apoptosis
    • Intracellular
    • p53 Pathway
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • p53
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • p53
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • p53 pathway
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab202689)
    • Anti-p53 (acetyl K382) antibody [EPR358(2)] - BSA and Azide free (ab219727)
  • Assay kits

    • p53 Transcription Factor Assay Kit (Colorimetric) (ab207225)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human p53 protein (ab43615)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab75754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
1/250 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ICC/IF
1/100 - 1/250.
Flow Cyt
1/20.
Notes
WB
1/250 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ICC/IF
1/100 - 1/250.
Flow Cyt
1/20.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
    • Tissue specificity

      Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.
    • Involvement in disease

      Note=TP53 is found in increased amounts in a wide variety of transformed cells. TP53 is frequently mutated or inactivated in about 60% of cancers. TP53 defects are found in Barrett metaplasia a condition in which the normally stratified squamous epithelium of the lower esophagus is replaced by a metaplastic columnar epithelium. The condition develops as a complication in approximately 10% of patients with chronic gastroesophageal reflux disease and predisposes to the development of esophageal adenocarcinoma.
      Defects in TP53 are a cause of esophageal cancer (ESCR) [MIM:133239].
      Defects in TP53 are a cause of Li-Fraumeni syndrome (LFS) [MIM:151623]. LFS is an autosomal dominant familial cancer syndrome that in its classic form is defined by the existence of a proband affected by a sarcoma before 45 years with a first degree relative affected by any tumor before 45 years and another first degree relative with any tumor before 45 years or a sarcoma at any age. Other clinical definitions for LFS have been proposed (PubMed:8118819 and PubMed:8718514) and called Li-Fraumeni like syndrome (LFL). In these families affected relatives develop a diverse set of malignancies at unusually early ages. Four types of cancers account for 80% of tumors occurring in TP53 germline mutation carriers: breast cancers, soft tissue and bone sarcomas, brain tumors (astrocytomas) and adrenocortical carcinomas. Less frequent tumors include choroid plexus carcinoma or papilloma before the age of 15, rhabdomyosarcoma before the age of 5, leukemia, Wilms tumor, malignant phyllodes tumor, colorectal and gastric cancers.
      Defects in TP53 are involved in head and neck squamous cell carcinomas (HNSCC) [MIM:275355]; also known as squamous cell carcinoma of the head and neck.
      Defects in TP53 are a cause of lung cancer (LNCR) [MIM:211980].
      Defects in TP53 are a cause of choroid plexus papilloma (CPLPA) [MIM:260500]. Choroid plexus papilloma is a slow-growing benign tumor of the choroid plexus that often invades the leptomeninges. In children it is usually in a lateral ventricle but in adults it is more often in the fourth ventricle. Hydrocephalus is common, either from obstruction or from tumor secretion of cerebrospinal fluid. If it undergoes malignant transformation it is called a choroid plexus carcinoma. Primary choroid plexus tumors are rare and usually occur in early childhood.
      Defects in TP53 are a cause of adrenocortical carcinoma (ADCC) [MIM:202300]. ADCC is a rare childhood tumor of the adrenal cortex. It occurs with increased frequency in patients with the Beckwith-Wiedemann syndrome and is a component tumor in Li-Fraumeni syndrome.
    • Sequence similarities

      Belongs to the p53 family.
    • Domain

      The nuclear export signal acts as a transcriptional repression domain. The TADI and TADII motifs (residues 17 to 25 and 48 to 56) correspond both to 9aaTAD motifs which are transactivation domains present in a large number of yeast and animal transcription factors.
    • Post-translational
      modifications

      Acetylated. Acetylation of Lys-382 by CREBBP enhances transcriptional activity. Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence.
      Phosphorylation on Ser residues mediates transcriptional activation. Phosphorylated by HIPK1 (By similarity). Phosphorylation at Ser-9 by HIPK4 increases repression activity on BIRC5 promoter. Phosphorylated on Thr-18 by VRK1. Phosphorylated on Ser-20 by CHEK2 in response to DNA damage, which prevents ubiquitination by MDM2. Phosphorylated on Thr-55 by TAF1, which promotes MDM2-mediated degradation. Phosphorylated on Ser-46 by HIPK2 upon UV irradiation. Phosphorylation on Ser-46 is required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated on Ser-15 upon ultraviolet irradiation; which is enhanced by interaction with BANP.
      Dephosphorylated by PP2A-PPP2R5C holoenzyme at Thr-55. SV40 small T antigen inhibits the dephosphorylation by the AC form of PP2A.
      May be O-glycosylated in the C-terminal basic region. Studied in EB-1 cell line.
      Ubiquitinated by MDM2 and SYVN1, which leads to proteasomal degradation. Ubiquitinated by RFWD3, which works in cooperation with MDM2 and may catalyze the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. Ubiquitinated by MKRN1 at Lys-291 and Lys-292, which leads to proteasomal degradation. Deubiquitinated by USP10, leading to its stabilization. Ubiquitinated by TRIM24, which leads to proteasomal degradation. Ubiquitination by TOPORS induces degradation. Deubiquitination by USP7, leading to stabilization. Isoform 4 is monoubiquitinated in an MDM2-independent manner.
      Monomethylated at Lys-372 by SETD7, leading to stabilization and increased transcriptional activation. Monomethylated at Lys-370 by SMYD2, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity. Lys-372 monomethylation prevents interaction with SMYD2 and subsequent monomethylation at Lys-370. Dimethylated at Lys-373 by EHMT1 and EHMT2. Monomethylated at Lys-382 by SETD8, promoting interaction with L3MBTL1 and leading to repress transcriptional activity. Demethylation of dimethylated Lys-370 by KDM1A prevents interaction with TP53BP1 and represses TP53-mediated transcriptional activation.
      Sumoylated by SUMO1.
    • Cellular localization

      Cytoplasm; Cytoplasm. Nucleus. Nucleus > PML body. Endoplasmic reticulum. Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2; Nucleus. Cytoplasm. Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli; Nucleus. Cytoplasm. Localized in the nucleus in most cells but found in the cytoplasm in some cells; Nucleus. Cytoplasm. Localized mainly in the nucleus with minor staining in the cytoplasm; Nucleus. Cytoplasm. Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4 and Nucleus. Cytoplasm. Predominantly nuclear but translocates to the cytoplasm following cell stress.
    • Target information above from: UniProt accession P04637 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7157 Human
      • Omim: 191170 Human
      • SwissProt: P04637 Human
      • Unigene: 654481 Human
      • Alternative names

        • Antigen NY-CO-13 antibody
        • BCC7 antibody
        • Cellular tumor antigen p53 antibody
        • FLJ92943 antibody
        • LFS1 antibody
        • Mutant tumor protein 53 antibody
        • p53 antibody
        • p53 tumor suppressor antibody
        • P53_HUMAN antibody
        • Phosphoprotein p53 antibody
        • Tp53 antibody
        • Transformation related protein 53 antibody
        • TRP53 antibody
        • tumor antigen p55 antibody
        • Tumor protein 53 antibody
        • Tumor protein p53 antibody
        • Tumor suppressor p53 antibody
        see all

      Images

      • Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
        Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
        All lanes : Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754) at 1/1000 dilution (Purified)

        Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
        Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) was starved overnight, then treatment with 30ug/ml etoposide for 8 hours followed by 500ng/ml Trichostatin A for 4 hours whole cell lysates

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 53 kDa
        Observed band size: 53 kDa



        p53 K382 acetylation is responsed to cell stress such as DNA damage caused by ultraviolet or ionizing radiation as was described in PMID 9744860 and 11250899

      • Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
        Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
        All lanes : Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754) at 1/1000 dilution (Purified)

        Lane 1 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
        Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) was starved overnight, then treatment with 30ug/ml etoposide for 8 hours followed by 500ng/ml Trichostatin A for 4 hours whole cell lysates

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 53 kDa
        Observed band size: 53 kDa



        p53 K382 acetylation is responsed to cell stress such as DNA damage caused by ultraviolet or ionizing radiation as was described in PMID 9744860 and 11250899

      • Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
        Western blot - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
        All lanes : Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754) at 1/5000 dilution (unpurified)

        Lane 1 : HepG2 cell lysates un-treated
        Lane 2 : HepG2 cell lysates treated with etopside and TSA

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 53 kDa
        Observed band size: 53 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
        Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)

        Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) treated with 500ng/ml Trichostatin A for 4 hours cells labeling p53 with purified ab75754 at 1/250 dilution (0.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      • Flow Cytometry - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
        Flow Cytometry - Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)

        Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) starved overnight, then treated with 30ug/ml etoposide for 8 hours followed by 500ng/ml TSA for 4 hours cells labeling p53 with purified ab75754 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

      • Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)
        Anti-p53 (acetyl K382) antibody [EPR358(2)] (ab75754)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (29)

      Publishing research using ab75754? Please let us know so that we can cite the reference in this datasheet.

      ab75754 has been referenced in 29 publications.

      • Ke X  et al. Heterogeneous Responses of Gastric Cancer Cell Lines to Tenovin-6 and Synergistic Effect with Chloroquine. Cancers (Basel) 12:N/A (2020). PubMed: 32033497
      • Zhang S  et al. MicroRNA-92a Targets SERTAD3 and Regulates the Growth, Invasion, and Migration of Prostate Cancer Cells via the P53 Pathway. Onco Targets Ther 13:5495-5514 (2020). PubMed: 32606766
      • Friedrich D  et al. Stochastic transcription in the p53-mediated response to DNA damage is modulated by burst frequency. Mol Syst Biol 15:e9068 (2019). PubMed: 31885199
      • Zhao J  et al. SIRT7 regulates hepatocellular carcinoma response to therapy by altering the p53-dependent cell death pathway. J Exp Clin Cancer Res 38:252 (2019). PubMed: 31196136
      • Lamichane S  et al. MHY2233 Attenuates Replicative Cellular Senescence in Human Endothelial Progenitor Cells via SIRT1 Signaling. Oxid Med Cell Longev 2019:6492029 (2019). PubMed: 31223423
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-6 of 6 Abreviews or Q&A

      Western blot abreview for Anti-p53 (acetyl K382) antibody [EPR358(2)]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      20 µg
      Gel Running Conditions
      Reduced Denaturing (13%)
      Sample
      Human Cell lysate - whole cell (HCT116 human colon carcinoma cell line)
      Specification
      HCT116 human colon carcinoma cell line
      Treatment
      DMSO (Ctl) and 10 ´M irinotecan (CPT-11) treatment for 24 hrs
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Mr. Christian Marx

      Verified customer

      Submitted Nov 03 2014

      Question

      Is the blocking peptide for this antibody available for purchase?

      Read More

      Abcam community

      Verified customer

      Asked on Dec 18 2013

      Answer

      We have added the blocking peptide for ab75754 as ab183615 to the catalog:
      https://www.abcam.com/p53-acetyl-k382-peptide-ab183615.html
      Our recommended peptide blocking study protocol is given through the link below as well:
      https://www.abcam.com/index.html?pageconfig=resource&rid=11378

      Read More

      Kevin Hanson

      Abcam Scientific Support

      Answered on Dec 18 2013

      Western blot abreview for Anti-p53 (acetyl K382) antibody [EPR358(2)]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (human fibroblast cell)
      Loading amount
      50 µg
      Specification
      human fibroblast cell
      Treatment
      TSA 500nM 24hr
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 05 2012

      Question

      Dear Ladies and Gentlemen,    I am interested in the acetyl-p53 antibody ab75754 by your company. On your website you say that ICC-application of this antibody was also tested, but I could not find an ICC-picture; could you send one by email, if possible?   Thank you, best regards,     

      Read More

      Abcam community

      Verified customer

      Asked on Dec 12 2011

      Answer

      Thank you for your inquiry. I am sorry to confirm that at the moment there is no ICC image available. But I would like to confirm that this antibody has been tested with HELA cells in ICC. I also would like to reassure you that this antibody is tested and therefore guaranteed to work in ICC. Please see our Abpromise® Guarantee summarized below: • 100% Scientific and Customer Support for any product you buy from Abcam or one of its authorized distributors. • We guarantee our products work in the tested species and applications as stated on the datasheet. • We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase. • We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards Please note these limitations to our Abpromise®: • Protocol information must be provided for the claim to be validated. • Antibodies tested in recombinant samples for use on endogenous samples are not covered. • “Predicted to react” information on the datasheets is provided for reference only. • Fast Track antibodies are covered for ELISA against the immunizing peptide only. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Dec 12 2011

      Western blot abreview for Anti-p53 (acetyl K382) antibody [EPR358(2)]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (SAEC)
      Loading amount
      35 µg
      Specification
      SAEC
      Treatment
      10uM Nutlin for 12hrs and virus infection
      Gel Running Conditions
      Reduced Denaturing (4-20%)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 29 2011

      Western blot abreview for Anti-p53 (acetyl K382) antibody [EPR358(2)]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HepG2)
      Loading amount
      20 µg
      Specification
      HepG2
      Treatment
      25 nM TCDD 24h then 75 µM Etoposide for 24h
      Gel Running Conditions
      Non-reduced Denaturing (12)
      Blocking step
      I-block as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
      Read More

      Dr. M Aggerbeck

      Verified customer

      Submitted Nov 26 2010

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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