Western blot abreview for Anti-p53 antibody

Good
Abreviews
Application
Western blot
Loading amount
75 µg
Gel Running Conditions
Reduced Denaturing (12.5%)
Sample
Rat Cell lysate - other (Gonad)
Specification
Gonad
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
6 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
Pierce ECL Femto
Bands
Specific: 53 kDa Non-specific: around 65, 72 kDa
Exposure
5 minute(s) and 0 second(s)

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Submitted Nov 10 2014

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