Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p53 antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Enzymatic
Sample
Rat Tissue sections (Gonad/oviduct)
Specification
Gonad/oviduct
Permeabilization
No
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
Protease-PBS solution (100mg protease/200ml PBS) at 37'C, 4mins - antigen retrieval. Tissue staining - using the VectaStain Elite ABC system

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Submitted Jan 30 2015

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