Western blot abreview for Anti-p53 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Epithelial OVCA cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
100 µg
Treatment
perifosine
Specification
Epithelial OVCA cell line
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
4 hour(s) and 0 minute(s)
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL Western Blotting Substrate
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 53 kDa

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Submitted Jul 18 2016

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