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  1. Link

    p53-antibody-pab-1801-ab28.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors p53
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Validated using a knockout cell line

Anti-p53 antibody [PAb 1801] (ab28)

  • Datasheet
  • SDS
Reviews (13)Q&A (6)References (57)

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Western blot - Anti-p53 antibody [PAb 1801] (ab28)
  • Western blot - Anti-p53 antibody [PAb 1801] (ab28)
  • ELISA - Anti-p53 antibody [PAb 1801] (ab28)
  • Western blot - Anti-p53 antibody [PAb 1801] (ab28)
  • Western blot - Anti-p53 antibody [PAb 1801] (ab28)

Key features and details

  • Mouse monoclonal [PAb 1801] to p53
  • Suitable for: WB, ELISA
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-p53 antibody [PAb 1801]
    See all p53 primary antibodies
  • Description

    Mouse monoclonal [PAb 1801] to p53
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    aa 46-55 of human p53
  • Positive control

    • WB: A431 cell lysate, MDA-MB-231 cell lysate.
  • General notes

    This monoclonal p53 antibody has been knockout validated in Western blot. The expected band for p53 was observed in HCT116 cells treated with irinotecan and the band was not seen in TP53 knockout HCT116 cell lysate.

    We recommend using ab1101 or ab154036, alternative mouse monoclonal antibodies, to detect human p53 by IHC, ICC/IF or flow cytometry since they perform better in these applications.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    PAb 1801
  • Myeloma

    NS1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • p53
    • Cell Biology
    • Apoptosis
    • Intracellular
    • p53 Pathway
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • p53
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • p53
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • p53 pathway
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Assay kits

    • p53 Transcription Factor Assay Kit (Colorimetric) (ab207225)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human p53 protein (ab43615)
  • Related Products

    • Genipin, Cell-permeable uncoupling protein 2 inhibitor (ab141049)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab28 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (7)
Use a concentration of 5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

We recommend using 3% milk as the blocking agent for Western blot.

ELISA
Use at an assay dependent concentration.
Notes
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

We recommend using 3% milk as the blocking agent for Western blot.

ELISA
Use at an assay dependent concentration.

Target

  • Function

    Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
  • Tissue specificity

    Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.
  • Involvement in disease

    Note=TP53 is found in increased amounts in a wide variety of transformed cells. TP53 is frequently mutated or inactivated in about 60% of cancers. TP53 defects are found in Barrett metaplasia a condition in which the normally stratified squamous epithelium of the lower esophagus is replaced by a metaplastic columnar epithelium. The condition develops as a complication in approximately 10% of patients with chronic gastroesophageal reflux disease and predisposes to the development of esophageal adenocarcinoma.
    Defects in TP53 are a cause of esophageal cancer (ESCR) [MIM:133239].
    Defects in TP53 are a cause of Li-Fraumeni syndrome (LFS) [MIM:151623]. LFS is an autosomal dominant familial cancer syndrome that in its classic form is defined by the existence of a proband affected by a sarcoma before 45 years with a first degree relative affected by any tumor before 45 years and another first degree relative with any tumor before 45 years or a sarcoma at any age. Other clinical definitions for LFS have been proposed (PubMed:8118819 and PubMed:8718514) and called Li-Fraumeni like syndrome (LFL). In these families affected relatives develop a diverse set of malignancies at unusually early ages. Four types of cancers account for 80% of tumors occurring in TP53 germline mutation carriers: breast cancers, soft tissue and bone sarcomas, brain tumors (astrocytomas) and adrenocortical carcinomas. Less frequent tumors include choroid plexus carcinoma or papilloma before the age of 15, rhabdomyosarcoma before the age of 5, leukemia, Wilms tumor, malignant phyllodes tumor, colorectal and gastric cancers.
    Defects in TP53 are involved in head and neck squamous cell carcinomas (HNSCC) [MIM:275355]; also known as squamous cell carcinoma of the head and neck.
    Defects in TP53 are a cause of lung cancer (LNCR) [MIM:211980].
    Defects in TP53 are a cause of choroid plexus papilloma (CPLPA) [MIM:260500]. Choroid plexus papilloma is a slow-growing benign tumor of the choroid plexus that often invades the leptomeninges. In children it is usually in a lateral ventricle but in adults it is more often in the fourth ventricle. Hydrocephalus is common, either from obstruction or from tumor secretion of cerebrospinal fluid. If it undergoes malignant transformation it is called a choroid plexus carcinoma. Primary choroid plexus tumors are rare and usually occur in early childhood.
    Defects in TP53 are a cause of adrenocortical carcinoma (ADCC) [MIM:202300]. ADCC is a rare childhood tumor of the adrenal cortex. It occurs with increased frequency in patients with the Beckwith-Wiedemann syndrome and is a component tumor in Li-Fraumeni syndrome.
  • Sequence similarities

    Belongs to the p53 family.
  • Domain

    The nuclear export signal acts as a transcriptional repression domain. The TADI and TADII motifs (residues 17 to 25 and 48 to 56) correspond both to 9aaTAD motifs which are transactivation domains present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications

    Acetylated. Acetylation of Lys-382 by CREBBP enhances transcriptional activity. Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence.
    Phosphorylation on Ser residues mediates transcriptional activation. Phosphorylated by HIPK1 (By similarity). Phosphorylation at Ser-9 by HIPK4 increases repression activity on BIRC5 promoter. Phosphorylated on Thr-18 by VRK1. Phosphorylated on Ser-20 by CHEK2 in response to DNA damage, which prevents ubiquitination by MDM2. Phosphorylated on Thr-55 by TAF1, which promotes MDM2-mediated degradation. Phosphorylated on Ser-46 by HIPK2 upon UV irradiation. Phosphorylation on Ser-46 is required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated on Ser-15 upon ultraviolet irradiation; which is enhanced by interaction with BANP.
    Dephosphorylated by PP2A-PPP2R5C holoenzyme at Thr-55. SV40 small T antigen inhibits the dephosphorylation by the AC form of PP2A.
    May be O-glycosylated in the C-terminal basic region. Studied in EB-1 cell line.
    Ubiquitinated by MDM2 and SYVN1, which leads to proteasomal degradation. Ubiquitinated by RFWD3, which works in cooperation with MDM2 and may catalyze the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. Ubiquitinated by MKRN1 at Lys-291 and Lys-292, which leads to proteasomal degradation. Deubiquitinated by USP10, leading to its stabilization. Ubiquitinated by TRIM24, which leads to proteasomal degradation. Ubiquitination by TOPORS induces degradation. Deubiquitination by USP7, leading to stabilization. Isoform 4 is monoubiquitinated in an MDM2-independent manner.
    Monomethylated at Lys-372 by SETD7, leading to stabilization and increased transcriptional activation. Monomethylated at Lys-370 by SMYD2, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity. Lys-372 monomethylation prevents interaction with SMYD2 and subsequent monomethylation at Lys-370. Dimethylated at Lys-373 by EHMT1 and EHMT2. Monomethylated at Lys-382 by SETD8, promoting interaction with L3MBTL1 and leading to repress transcriptional activity. Demethylation of dimethylated Lys-370 by KDM1A prevents interaction with TP53BP1 and represses TP53-mediated transcriptional activation.
    Sumoylated by SUMO1.
  • Cellular localization

    Cytoplasm; Cytoplasm. Nucleus. Nucleus > PML body. Endoplasmic reticulum. Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2; Nucleus. Cytoplasm. Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli; Nucleus. Cytoplasm. Localized in the nucleus in most cells but found in the cytoplasm in some cells; Nucleus. Cytoplasm. Localized mainly in the nucleus with minor staining in the cytoplasm; Nucleus. Cytoplasm. Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4 and Nucleus. Cytoplasm. Predominantly nuclear but translocates to the cytoplasm following cell stress.
  • Target information above from: UniProt accession P04637 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7157 Human
    • Omim: 191170 Human
    • SwissProt: P04637 Human
    • Unigene: 654481 Human
    • Alternative names

      • Antigen NY-CO-13 antibody
      • BCC7 antibody
      • Cellular tumor antigen p53 antibody
      • FLJ92943 antibody
      • LFS1 antibody
      • Mutant tumor protein 53 antibody
      • p53 antibody
      • p53 tumor suppressor antibody
      • P53_HUMAN antibody
      • Phosphoprotein p53 antibody
      • Tp53 antibody
      • Transformation related protein 53 antibody
      • TRP53 antibody
      • tumor antigen p55 antibody
      • Tumor protein 53 antibody
      • Tumor protein p53 antibody
      • Tumor suppressor p53 antibody
      see all

    Images

    • Western blot - Anti-p53 antibody [PAb 1801] (ab28)
      Western blot - Anti-p53 antibody [PAb 1801] (ab28)
      All lanes : Anti-p53 antibody [PAb 1801] (ab28) at 5 µg/ml

      Lane 1 : Wild-type HCT116 cell lysate at 30 µg
      Lane 2 : Wild-type HCT116 + irinotecan (10 µM, 24 hours) cell lysate at 30 µg
      Lane 3 : p53 knockout HCT116 cell lysate at 30 µg
      Lane 4 : p53 knockout HCT116 + irinotecan (10 µM, 24 hours) cell lysate at 30 µg
      Lane 5 : A431 cell lysate (positive control) at 20 µg
      Lane 6 : Saos-2 cell lysate (negative control) at 20 µg
      Lane 7 : MEF cell lysate at 20 µg
      Lane 8 : Wild-type HAP1 cell lysate at 20 µg
      Lane 9 : p53 knockout HAP1 cell lysate at 20 µg

      Performed under reducing conditions.

      Predicted band size: 53 kDa



      Lanes 1-9: Merged (red and green) signal.


      Ab28 was shown to specifically react with p53 in wild-type HAP1 and HCT116 cells treated with irinotecan. No band was observed in p53 knockout HAP1 or HCT116 cells. Wild-type and p53 knockout samples, positive and negative controls were subjected to SDS-PAGE. Ab28 and ab181602(loading control to GAPDH) were diluted 5 μg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-p53 antibody [PAb 1801] (ab28)
      Western blot - Anti-p53 antibody [PAb 1801] (ab28)
      Anti-p53 antibody [PAb 1801] (ab28) at 5 µg/ml + MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 53 kDa
      Additional bands at: 53 kDa, 60 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes


      This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab28 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.

       

      We recommend using 3% milk as the blocking agent for Western blot.

    • ELISA - Anti-p53 antibody [PAb 1801] (ab28)
      ELISA - Anti-p53 antibody [PAb 1801] (ab28)
      Analysis of recombinant p53 protein (ab43615) in MCF7 cells treated with vehicle (VEH)as well as MCF7 cells treated with camptothecin (CAM) and no material (NO) by sandwich ELISA with the use of anti-53 antibody (ab28) as capture and anti-p53 antibody (ab32389) as detector.
    • Western blot - Anti-p53 antibody [PAb 1801] (ab28)
      Western blot - Anti-p53 antibody [PAb 1801] (ab28)

      All Lanes: Anti-p53 antibody [PAb 1801] (ab28) at 5 µg/ml

      Lane 1 and 10: Wild-type HCT116 cell lysate

      Lane 2 and 11: Wild-type HCT116 + irinotecan (10 µM, 24 hours) cell lysate

      Lane 3 and 12: p53 knockout HCT116 cell lysate

      Lane 4 and 13: p53 knockout HCT116 + irinotecan (10 µM, 24 hours) cell lysate

      Lane 5 and 14: A431 cell lysate (positive control)

      Lane 6 and 15: Saos-2 cell lysate (negative control)

      Lane 7 and 16: MEF cell lysate

      Lane 8 and 17: Wild-type HAP1 cell lysate

      Lane 9 and 18: p53 knockout HAP1 cell lysate

      Lanes 1-9: 1% BSA blocking buffer

      Lanes 10-18: 3% Milk blocking buffer

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) at 1/5000 dilution

      We recommend using 3% milk as the blocking agent for western blot.

    • Western blot - Anti-p53 antibody [PAb 1801] (ab28)
      Western blot - Anti-p53 antibody [PAb 1801] (ab28)

      The image shows a Western blot for ab28, testing the threshold detection of the p53 antibody. The lanes are as follows:

      Lane 1 - 20ug of A549 lysate, lane 2 - 10ug of A549 lysate, lane 3 - 5ug of A549 lysate, lane 4 - 2.5ug of A549 lysate, lane 5 - 1.5ug of A549 lysate.

      This picture was submitted as part of the review by Craig Carson.

       

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (57)

    Publishing research using ab28? Please let us know so that we can cite the reference in this datasheet.

    ab28 has been referenced in 57 publications.

    • Wang Y  et al. Carboxypeptidase A4 negatively correlates with p53 expression and regulates the stemness of breast cancer cells. Int J Med Sci 18:1753-1759 (2021). PubMed: 33746592
    • Pennacchiotti G  et al. SPINK7 expression changes accompanied by HER2, P53 and RB1 can be relevant in predicting oral squamous cell carcinoma at a molecular level. Sci Rep 11:6939 (2021). PubMed: 33767253
    • Uhlmann C  et al. Efficient Modulation of TP53 Expression in Human Induced Pluripotent Stem Cells. Curr Protoc Stem Cell Biol 52:e102 (2020). PubMed: 31883435
    • Keshri GK  et al. Effects of Pulsed 810?nm Al-Ga-As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight. Lasers Surg Med 52:424-436 (2020). PubMed: 31483061
    • Lee AS  et al. Detection of a High Ratio of Soluble to Membrane-Bound LOX-1 in Aspirated Coronary Thrombi From Patients With ST-Segment-Elevation Myocardial Infarction. J Am Heart Assoc 9:e014008 (2020). PubMed: 31928155
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 13 Abreviews

    Immunocytochemistry abreview for Anti-p53 antibody [PAb 1801]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (SHSY5Y cells)
    Permeabilization
    Yes - TritonX-100 in PBS 10min
    Specification
    SHSY5Y cells
    Blocking step
    (agent) for 20 minute(s) · Concentration: 100%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 09 2020

    Western blot abreview for Anti-p53 antibody [PAb 1801]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SHSY5Y cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% tris glycin gel)
    Loading amount
    40 µg
    Specification
    SHSY5Y cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 31 2020

    Western blot abreview for Anti-p53 antibody [PAb 1801]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (CT26 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% tris glycin gels)
    Loading amount
    40 µg
    Specification
    CT26 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 31 2020

    Western blot abreview for Anti-p53 antibody [PAb 1801]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Colon cancer cell line)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    30 µg
    Treatment
    10 µM CPT-11 for 24 hrs
    Specification
    Colon cancer cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Anke Rauch

    Verified customer

    Submitted Jun 28 2016

    Western blot abreview for Anti-p53 antibody [PAb 1801]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Colon lysate)
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Loading amount
    20 µg
    Treatment
    NoTreated and DSS-treated
    Specification
    Colon lysate
    Blocking step
    Milk as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Natalia Volodko

    Verified customer

    Submitted Aug 07 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-p53 antibody [PAb 1801]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human medulloblastoma cell line D556)
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Human medulloblastoma cell line D556
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 11 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p53 antibody [PAb 1801]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate
    Sample
    Mouse Tissue sections (mouse brain cerebellum)
    Specification
    mouse brain cerebellum
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 23 2015

    Western blot abreview for Anti-p53 [PAb 1801] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (mouse cerebellum)
    Specification
    mouse cerebellum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    Submitted Apr 03 2014

    Immunoprecipitation abreview for Anti-p53 [PAb 1801] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A/G
    Sample
    Human Cell lysate - whole cell (Human 293T cells)
    Specification
    Human 293T cells
    Total protein in input
    200 µg
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 31 2014

    Western blot abreview for Anti-p53 antibody [PAb 1801]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human glioblastoma cell lines)
    Loading amount
    120 µg
    Specification
    human glioblastoma cell lines
    Gel Running Conditions
    Reduced Denaturing (12,5%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 16 2011

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