For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
At the moment I am trying to investigate the p53 activity in my cell line using the western blot technique. I am using a antibody for total p53 and a phospho-p53 (Ser15) antibody. I ordered those antibodies from a different company. However I am not completely happy with those antibodies. Therefore I am looking into ordering new antibodies.
I was wondering which are the conditions you optimised your p53 antibody (ab26 or ab28) and phospho-p53 (Ser15) antibody (amount of protein needed, Blotiing solution and time, etc.)? How long would the shipping be taking?
Asked on Mar 16 2012
Thank you for contacting us.
Our p53 antibody ab26 has been optimized by loading 20 ug per lane of HeLa cells, using 5% BSA as the blocking agent for 1 hour at room temperature, and incubating with the primary antibody at a concentration of 5ug / mL overnight at 4C. To view the protocols used by our customers, you can browse our WB Abreviews for this product:
Ab28 and ab1431 can be used with a similar protocol, loading 20-40 ug mer lane, using a 5% milk block, and a primary antibody dilution of 1:1000.
We will ship anywhere in the UK or US overnight for next day delivery. I hope this helps, please let me know if you need any additional information or assistance.
Answered on Mar 16 2012