Question (4338) | Anti-p53 antibody [PAb 1801] (ab28)

Go to datasheet (ab28)

Question

Using this Ab, I get a distinct band at about 53kD and one at about 73KD with minor bands at less than 50Kd. Does this Ab recognize p73 or other proteins?

Answer

It is very common to see multiple bands when using an antibody to p53. Your band at 73kDa is almost certainly p73 as this protein shows sequence homology to p53 and will be recognised by this antibody. Other bands may be due to the different modificaiton states of p53, although we'd typically expect these to run at over 53 kDa.

Sign up