Western blot abreview for Anti-p53 [PAb 1801] antibody

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (mouse cerebellum)
Specification
mouse cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: 53 kDa
Exposure
1 second(s)

Additional data

Additional Notes
p53 protein level is determined in two 7-day-old mouse pup cerebellum.

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Submitted Apr 03 2014

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