Non-Abcam antibody was used: GE healthcareLNA931V/AG Host species: Mouse Clonality: Polyclonal Conjugation: Horse Radish Peroxidase
10 minute(s) and 0 second(s)
Specific: 53 kDa Non-specific: 40 kDa
We always use abcam p53 antibodies for mouse colon lysates and we were happy with them. But the newly purchased lot GR127782-3 doesn't seem to work that well - it gave me smudged bands. I attached my results with the new lot (GR127782-3) and the old GR127782-1 lot, so you can see the difference. I don't think that's a gel or transfer problem, since I cut the membrane and blotted lower part with different antibodies and the bans were nice. And I did it two times and they look the same. I am wondering if I can be caused by antibodies.
These 2 lots are from the same batch material. This issue was investigated. Unfortunately, the customer may have received a faulty vial and was offered a free of charge replacement.
Dr. Natalia Volodko
Submitted Aug 07 2015
Get resources and offers direct to your inboxSign up