Western blot abreview for Anti-p53 antibody [PAb 1801]

Below Average
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Colon lysate)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
20 µg
Treatment
NoTreated and DSS-treated
Specification
Colon lysate
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: GE healthcareLNA931V/AG
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 53 kDa Non-specific: 40 kDa

Additional data

Additional Notes
We always use abcam p53 antibodies for mouse colon lysates and we were happy with them. But the newly purchased lot GR127782-3 doesn't seem to work that well - it gave me smudged bands. I attached my results with the new lot (GR127782-3) and the old GR127782-1 lot, so you can see the difference. I don't think that's a gel or transfer problem, since I cut the membrane and blotted lower part with different antibodies and the bans were nice. And I did it two times and they look the same. I am wondering if I can be caused by antibodies.
Abcam response
These 2 lots are from the same batch material. This issue was investigated. Unfortunately, the customer may have received a faulty vial and was offered a free of charge replacement.

Dr. Natalia Volodko

Verified customer

Submitted Aug 07 2015

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