Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p53 antibody [PAb 1801]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate
Sample
Mouse Tissue sections (mouse brain cerebellum)
Specification
mouse brain cerebellum
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/250

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse 2nd Ab
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/500

Additional data

Additional Notes
p53 was stained in a mouse model of medulloblastoma. The picture shows the strong staining in the center of the tumor.

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Submitted Feb 23 2015

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