Immunocytochemistry/ Immunofluorescence abreview for Anti-p53 antibody [PAb 1801]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human medulloblastoma cell line D556)
Permeabilization
Yes - 0.3% Triton X-100
Specification
Human medulloblastoma cell line D556
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/250

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Alexa Fluor 555 goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Abcam user community

Verified customer

Submitted May 11 2015

Sign up