Western blot abreview for Anti-p53 antibody [PAb 1801]

Average
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Colon cancer cell line)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
30 µg
Treatment
10 µM CPT-11 for 24 hrs
Specification
Colon cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% Milk/ PBS-T
Dilution
1/2000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-Mouse IgG HRP-conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Super Signal West Pico (Pierce) ECL
Negative control
Lane 3: HCT-116 p53-/- cells, untreated (30 µg). Lane 4: HCT-116 p53-/- cells, CPT-11 treated (30 µg).
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 55, 51 kDa Non-specific: 45, 60, 80 kDa
Positive control
Lane 1: HCT-115 wild type cells, untreated (30 µg). Lane 2: HCT-116 wild type cells, CPT-11 treated (30 µg).

Anke Rauch

Verified customer

Submitted Jun 28 2016

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