For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Mouse Cell lysate - whole cell (Colon cancer cell line)
Gel Running Conditions
Reduced Denaturing (12% gel)
10 µM CPT-11 for 24 hrs
Colon cancer cell line
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% Milk/ PBS-T
Non-Abcam antibody was used: Anti-Mouse IgG HRP-conjugated
Host species: Goat
Conjugation: Horse Radish Peroxidase
Super Signal West Pico (Pierce) ECL
Lane 3: HCT-116 p53-/- cells, untreated (30 µg).
Lane 4: HCT-116 p53-/- cells, CPT-11 treated (30 µg).
5 minute(s) and 0 second(s)
Specific: 55, 51 kDa Non-specific: 45, 60, 80 kDa
Lane 1: HCT-115 wild type cells, untreated (30 µg).
Lane 2: HCT-116 wild type cells, CPT-11 treated (30 µg).
Submitted Jun 28 2016