Application
Western blot
Sample
Human Cell lysate - whole cell (Breast cancer cell-lines)
Loading amount
20 µg
Specification
Breast cancer cell-lines
Gel Running Conditions
Reduced Denaturing (4-20% Nupage gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% Milk in TBS-TWEEN
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse secondary
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
10 second(s)
Bands
Specific: 53 kDa Non-specific: 72 kDa
Positive control
Mutant MDA231 cell-line
Additional data
Additional Notes
1. MCF7 cells (p53 WT), 2. MDA231 cells (p53 Mutant)
The antibody gives a stronger band at the non-specific 75KDa size but can detect the 53KDa protein well.
The antibody gives a stronger band at the non-specific 75KDa size but can detect the 53KDa protein well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Cherie Blenkiron
Verified customer
Submitted Jul 18 2008