Application
Western blot
Sample
Human Cell lysate - whole cell (PNT2 prostate tissue)
Gel Running Conditions
Reduced Denaturing (7)
Loading amount
30 µg
Specification
PNT2 prostate tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TTBS + 5% BSA
Dilution
1/1000
Detection
Detection method
ECL+
Negative control
PNT2 cells transfected with siRNA against p53
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 50-53 kDa
Positive control
PNT2 cells transfected with mock siRNA
Additional data
Additional Notes
Lane 1: PNT2 cell line (WT)
Lanes 2-8: Clones derived from PNT2 cells treated with Cas9/sgRNA against p53
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Submitted Jan 08 2019