Application
Western blot
Sample
Human Cell lysate - whole cell (ovarian and colon)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Treatment
3.2uM of drug for various time points
Specification
ovarian and colon
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Dilution
1/400
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: peroxidase conjugated secondary antibodie
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
no exposure
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: ~50 kDa
Positive control
doxorubicin treated cells
Additional data
PubMed ID
27090292: View
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Abcam user community
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Submitted Aug 21 2019