Recombinant
RabMAb

Recombinant Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026)

Overview

  • Product name

    Anti-p53 DINP1/TP53INP1 antibody [EPR17974]
    See all p53 DINP1/TP53INP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR17974] to p53 DINP1/TP53INP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human p53 DINP1/TP53INP1 aa 100-200. The exact sequence is proprietary.
    Database link: Q96A56
    (Peptide available as ab206313)

  • Positive control

    • WB: HepG2, SK-BR-3 C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human pancreas lysate. IHC-P: Human colonic adenocarcinoma, mouse kidney and rat kidney tissues. ICC/IF: HepG2 cells.
  • General notes

     This product was previously labelled as p53 DINP1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab202026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/300.

Target

  • Function

    In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis.
  • Tissue specificity

    Ubiquitously expressed.
  • Cellular localization

    Nucleus > PML body.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp434M1317 antibody
    • FLJ22139 antibody
    • p53 damage inducible nuclear protein 1 antibody
    • p53 dependent damage inducible nuclear protein 1 antibody
    • p53 inducible nuclear protein 1 antibody
    • p53 inducible p53DINP1 antibody
    • p53-dependent damage-inducible nuclear protein 1 antibody
    • p53DINP1 antibody
    • SIP antibody
    • Stress induced protein antibody
    • Stress-induced protein antibody
    • T53I1_HUMAN antibody
    • Teap antibody
    • TP53 DINP1 antibody
    • TP53 INP1 antibody
    • TP53DINP1 antibody
    • TP53INP1 antibody
    • TP53INP1A antibody
    • TP53INP1B antibody
    • Tumor protein p53 inducible nuclear protein 1 antibody
    • Tumor protein p53-inducible nuclear protein 1 antibody
    see all

Images

  • All lanes : Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026) at 1/10000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 2 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa


    Exposure time: 30 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling p53 DINP1/TP53INP1 with ab202026 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing nuclear staining on HepG2 cells.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab202026 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026) at 1/10000 dilution + Human pancreas lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa


    Exposure time: 30 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • All lanes : Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling p53 DINP1/TP53INP1  with ab202026 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasm and nucleus staining on cancer cells of Human colonic adenocarcinoma is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling p53 DINP1/TP53INP1  with ab202026 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasm staining on epithelial cells of mouse kidney tubules is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling p53 DINP1/TP53INP1 with ab202026 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasm and weak nucleus staining on epithelial cells of rat kidney tubules is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Lu B  et al. SUMOylation Evoked by Oxidative Stress Reduced Lens Epithelial Cell Antioxidant Functions by Increasing the Stability and Transcription of TP53INP1 in Age-Related Cataracts. Oxid Med Cell Longev 2019:7898069 (2019). Read more (PubMed: 31281592) »
  • Kalinina TS  et al. Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro. Mol Biol Rep N/A:N/A (2018). Read more (PubMed: 30421125) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell: MCF-7)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Treatment
4ug P53 DINP1 reconstruction plasmid and siRNA for P53DINP1 for 48 hrs
Specification
breast cancer cell: MCF-7
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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Verified customer

Submitted Dec 30 2015

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