• Product name
  • Description
    Rabbit polyclonal to p53R2
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 2-17 of Human p53R2.

  • Positive control
    • A431 or 3T3 cell lysate



Our Abpromise guarantee covers the use of ab8105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).Can be blocked with p53R2 peptide (ab6237).
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 18997010


  • Function
    Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.
  • Tissue specificity
    Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma.
  • Pathway
    Genetic information processing; DNA replication.
  • Involvement in disease
    Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8A (MTDPS8A) [MIM:612075]. A disorder due to mitochondrial dysfunction characterized by various combinations of neonatal hypotonia, neurological deterioration, respiratory distress, lactic acidosis, and renal tubulopathy.
    Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8B (MTDPS8B) [MIM:612075]. A disease due to mitochondrial dysfunction and characterized by ophthalmoplegia, ptosis, gastrointestinal dysmotility, cachexia, peripheral neuropathy.
    Defects in RRM2B are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 5 (PEOA5) [MIM:613077]. A disorder characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
  • Sequence similarities
    Belongs to the ribonucleoside diphosphate reductase small chain family.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates from cytoplasm to nucleus in response to DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686M05248 antibody
    • MGC102856 antibody
    • MGC42116 antibody
    • MTDPS8A antibody
    • MTDPS8B antibody
    • p53 inducible ribonucleotide reductase small subunit 2 homolog antibody
    • p53 inducible ribonucleotide reductase small subunit 2 like protein antibody
    • P53 inducible ribonucleotide reductase small subunit 2 short form beta antibody
    • p53 R2 antibody
    • p53-inducible ribonucleotide reductase small subunit 2-like protein antibody
    • p53R2 antibody
    • Ribonucleoside diphosphate reductase M2 subunit B antibody
    • Ribonucleoside-diphosphate reductase subunit M2 B antibody
    • Ribonucleotide reductase M2 B (TP53 inducible) antibody
    • Ribonucleotide reductase M2 B antibody
    • Ribonucleotide reductase small subunit like 2 p53 inducible antibody
    • RIR2B_HUMAN antibody
    • RRM 2B antibody
    • RRM2B antibody
    • TP53 inducible ribonucleotide reductase M2 B antibody
    • TP53-inducible ribonucleotide reductase M2 B antibody
    see all


  • Anti-p53R2 antibody (ab8105) at 1/1000 dilution + U2OS whole cell lysate treated with UV irradiation.

    Goat anti-rabbit at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 39 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 150 kDa (possible cross reactivity)

    Exposure time: 1 minute

    Blot was incubated for one hour in 5% milk for blocking, and incubated in primary antibody for 16 hours.
  • All lanes : Anti-p53R2 antibody (ab8105) at 1 µg/ml

    Lane 1 : A431 cell lysate
    Lane 2 : 3T3 cell lysate with absence of blocking peptide
    Lane 3 : 3T3 cell lysate with presence of blocking peptide

    Predicted band size: 41 kDa

  • ab8105 at 1µg/ml staining p53R2 in lung tissue by IHC

  • ICC/IF image of ab8105 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8105, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of p53R2 in Human Lung cells using ab8105 at 20 ug/ml.


This product has been referenced in:
  • Chen J  et al. Overexpression of p53R2 is associated with poor prognosis in lung sarcomatoid carcinoma. BMC Cancer 17:855 (2017). Read more (PubMed: 29246119) »
  • Narayanaswamy PB  et al. Transcriptomic pathway analysis of urokinase receptor silenced breast cancer cells: a microarray study. Oncotarget 8:101572-101590 (2017). Read more (PubMed: 29254187) »
See all 22 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for your inquiry.

Since the working dilutions of antibodies are not a fixed value but have to optimised by the endusers themselves according to experimental condition, I cannot give you absolute numbers. Even when the pre-diluted antibodies are used neat there might be reasons to use 50ul per slide or maybe even 100ul per slide.

These conditions include: size of the sample, tissue type, lab experience, diluent buffer, species, antigen retrieval, incubation time, etc.

For the products you mentioned I can give a estimate for the pre-diluted antibodies of ca 140- 280slides, for the other antibodies 500 - 100 slides and for the detection kit 150 - 300 slides.

I hope this information is helpful.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (U2OS)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

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Verified customer

Submitted Sep 01 2005


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